| Wu Mengyun,Li Wei,Zhang Huadong,et al.Establishment and preliminary validation of a dose-response curve for semi-automated identification of nucleoplasmic bridges in human peripheral blood lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,2026,46(7):656-661 |
| Establishment and preliminary validation of a dose-response curve for semi-automated identification of nucleoplasmic bridges in human peripheral blood lymphocytes |
| Received:December 25, 2024 |
| DOI：10.3760/cma.j.cn112271-20241225-00495 |
| KeyWords:Nucleoplasmic bridge Automation Dose-response curve |
| FundProject:重庆市科卫联合医学科研项目(2022ZDXM034) |
| Author Name | Affiliation | E-mail | | Wu Mengyun | Institute of Public Health Security and Surveillance, Chongqing Center for Disease Control and Prevention, Chongqing 400042, China | | | Li Wei | Institute of Public Health Security and Surveillance, Chongqing Center for Disease Control and Prevention, Chongqing 400042, China | wumycdc@163.com | | Zhang Huadong | Institute of Public Health Security and Surveillance, Chongqing Center for Disease Control and Prevention, Chongqing 400042, China | | | Yuan Fang | Institute of Public Health Security and Surveillance, Chongqing Center for Disease Control and Prevention, Chongqing 400042, China | | | Tan Xiuhong | Institute of Public Health Security and Surveillance, Chongqing Center for Disease Control and Prevention, Chongqing 400042, China | |
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| Abstract:: |
| Objective To identify nucleoplasmic bridges in human peripheral blood lymphocytes induced by different radiation doses using automated scanning software, and establish a dose-response curve for nucleoplasmic bridges induced in human peripheral blood lymphocytes by 60Co γ-rays. Methods Twenty-four milliliters of heparin sodium-anticoagulated venous blood were collected from 4 healthy volunteers (2 males and 2 females), and irradiated in vitro with 0, 0.25, 0.51, 1, 2, 3, 4, and 5 Gy of 60Co γ-rays (dose rate 0.85 Gy/min). Samples were cultured and prepared using the cytokinesis-block method with cytochalasin B. The nucleoplasmic bridges in binucleated cells were recorded using automatic scanning and recognition software, and a dose-response curve was fitted based on the number of nucleoplasmic bridges per cell. The curve was then used to estimate the nucleoplasmic bridge-based biological dose of irradiated blind samples. Results The application for automatically scanning and identifying nucleoplasmic bridges boasted a repeatability, accuracy, and true positive rate all exceeding 98%, with a false positive rate below 50%. The repeatability, accuracy, and true positive rate of automatic scanning and identification of nucleoplasmic bridges were all higher than 98%, and the false positive rate was lower than 50%. Within the dose range of 0 to 5 Gy, the fitted dose-response curve for nucleoplasmic bridges conformed to a quadratic polynomial model. The regression equation was y=0.391 7 × 10-3D2 + 3.225 8 × 10-3D + 0.175 8 × 10-3 (R2=0.999), where D represents the dose. The relative deviations between the estimated doses of the validation samples and the actual irradiation doses were <20%. Conclusions A dose-response curve based on the semi-automated analysis of nucleoplasmic bridges was successfully constructed, and its feasibility for estimating biological doses was validated. |
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