| Dai Qingchi,Xi Cong,Yan Yukang,et al.Untargeted metabolomics-based selection and validation of radiation-sensitive metabolites in rat plasma[J].Chinese Journal of Radiological Medicine and Protection,2026,46(6):555-563 |
| Untargeted metabolomics-based selection and validation of radiation-sensitive metabolites in rat plasma |
| Received:December 24, 2025 |
| DOI：10.3760/cma.j.cn112271-20251224-00445 |
| KeyWords:Ionizing radiation|Untargeted metabolomics|Absolute quantification|Biomarker|Metabolic pathway |
| FundProject:国家自然科学基金(82003393);中国疾控中心辐射安全所青年科学研究所长基金(2023-02) |
| Author Name | Affiliation | E-mail | | Dai Qingchi | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Xi Cong | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Yan Yukang | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Li Shuang | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Cai Tianjing | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Wang Xiaorui | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Zhao Hua | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | zhaohua@nirp.chinacdc.cn |
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| Abstract:: |
| Objective To select and preliminarily validate radiation-sensitive metabolites in the plasma of rats exposed to total-body irradiation (TBI), aiming to provide an experimental basis for research on radiation biomarkers. Methods Models of rats subjected to TBI were established using 60Co γ-rays at doses of 0, 4, 8, and 12 Gy. Plasma samples were then collected from these rats at 6 h post-irradiation. Using untargeted metabolomics, differential metabolites were selected from the plasma samples, followed by pathway enrichment analysis. Stable metabolites were then identified and validated using the targeted absolute quantification. Results A total of 323 differential metabolites were identified through untargeted analysis, and they were primarily enriched in pathways such as pyrimidine metabolism. In combination with a literature review, three stable candidate metabolites were selected: taurine, hexanoylcarinitine, and butyrylcarnitine. Targeted absolute quantification result indicate that the three metabolites followed trends consistent with those observed in the untargeted analysis. Among these, taurine exhibited a dose-dependent upregulation, and the fitted dose-effect model was y = 2.337x + 37.54 (F = 6.76, P < 0.05). These metabolites all demonstrated high classification accuracy (AUC > 0.7) in discriminating between the 0 Gy and > 0 Gy irradiation groups. Conclusions Metabolites in rat plasma change significantly at 6 h post-TBI. As radiation-sensitive metabolites, taurine, hexanoylcarnitine, and butyrylcarnitine are relatively stable. Notably, taurine exhibits a clear dose-effect relationship. The combination of untargeted metabolomics-based selection and validation using targeted absolute quantification offers a reliable method for identifying radiation biomarkers. |
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