| Ba Xiaoyan,Li Shuyan,Zheng Ke,et al.Mechanistic research of B7 homolog 3-mediated radioresistance in glioblastoma[J].Chinese Journal of Radiological Medicine and Protection,2026,46(5):497-503 |
| Mechanistic research of B7 homolog 3-mediated radioresistance in glioblastoma |
| Received:October 18, 2025 |
| DOI：10.3760/cma.j.cn112271-20251018-00363 |
| KeyWords:B7-H3 Glioblastoma Radiosensitivity STAT3 |
| FundProject:河南省科技攻关计划项目(212102310663);河南省医学科技攻关(SBGJ202503016);国家自然科学基金(82302937) |
| Author Name | Affiliation | E-mail | | Ba Xiaoyan | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | | | Li Shuyan | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | | | Zheng Ke | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | | | Huang Rong | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | | | Sun Xueming | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | | | Wu Hui | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | wuhui7008@126.com |
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| Abstract:: |
| Objective To investigate the regulatory effect of B7 homolog 3 (B7-H3) on the radiosensitivity of glioblastoma and its potential mechanisms. Methods Human glioblastoma U251 cells were selected. B7-H3 overexpression, knockdown, and empty vector control groups were established via lentiviral transfection, and the infection efficiency was verified by q-PCR and Western blot. Colony formation assay was performed with 0, 2, 4, 6, and 8 Gy X-ray irradiation to calculate the survival fraction (SF) and sensitization enhancement ratio (SER) Cells were irradiated with 10 Gy X-rays for functional assays. Cell proliferation was assessed by CCK-8 assay at 24, 48, 72, and 96 h post-irradiation. Invasion ability was evaluated by Transwell assay, and apoptosis was detected by flow cytometry and Western blot at 48 h post-irradiation. The protein expression levels of B7-H3, Cleaved-Caspase-3, STAT3, and phosphorylated STAT3 (p-STAT3) were determined by Western blot. Results Stable cell lines with regulated B7-H3 expression were successfully established. Compared with the empty vector control group, cells in the overexpression group exhibited radioresistance (SF2=0.70, 0.60, P<0.01). Overexpression of B7-H3 attenuated the inhibitory effect of radiation on cell proliferation (48 h: t=4.40, P<0.01), promoted cell invasion (t=4.87, P<0.01), and decreased the apoptosis rate (t=5.75, P< 0.01). Western blot showed that Cleaved-Caspase-3 expression was significantly upregulated in the knockdown group (t=7.49, P<0.01). Radiation upregulated B7-H3 expression (t=5.57, P<0.01). B7-H3 overexpression could activate STAT3 phosphorylation (t=9.97, P< 0.01). Conclusions B7-H3 influences the radiosensitivity of GBM cells by regulating STAT3 phosphorylation, suggesting its potential as a novel target for improving the efficacy of radiotherapy in GBM. |
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