| Zheng Zexuan,Shen Jiahao,Pei Hailong,et al.PLIN5 enhances radioresistance in non-small cell lung cancer cells by mediating the accumulation of lipid droplets[J].Chinese Journal of Radiological Medicine and Protection,2026,46(5):483-489 |
| PLIN5 enhances radioresistance in non-small cell lung cancer cells by mediating the accumulation of lipid droplets |
| Received:September 18, 2025 |
| DOI：10.3760/cma.j.cn112271-20250918-00336 |
| KeyWords:Perilipin 5 Neutral lipids Non-small cell lung cancer Radiosensitivity |
| FundProject:国家自然科学基金(82273578) |
| Author Name | Affiliation | E-mail | | Zheng Zexuan | School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University, Suzhou 215123, China | | | Shen Jiahao | School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University, Suzhou 215123, China | | | Pei Hailong | School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University, Suzhou 215123, China | | | Li Wanshi | Zhejiang Cancer Hospital, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, China | liws@zjcc.org.cn |
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| Abstract:: |
| Objective To investigate the effect of perilipin 5 (PLIN5) on neutral lipid content and radiosensitivity in non-small cell lung cancer (NSCLC) cells and its underlying mechanism. Methods The NSCLC cell line A549 was irradiated with 0 or 2 Gy of X-rays. PLIN5 gene expression levels in the two groups were detected via differential gene expression analysis and quantitative real-time PCR (qPCR). Cell lines stably overexpressing full-length PLIN5 or a truncated mutant lacking the lipid-binding domain, as well as PLIN5 knockdown cell lines, were constructed. Wild-type cells and empty vector-transfected cells served as controls. These cell groups were irradiated with 0 Gy or 8 Gy of X-rays. Relative cell proliferation rate curve and cell apoptosis was assessed. At 24 h post-irradiation, cells were stained with BODIPY 594 and Hoechst, and observed under a confocal microscope to evaluate lipid expression levels. Results After 2 Gy X-ray irradiation, 487 differentially expressed genes were identified in A549 cells. qPCR validation showed that PLIN5 expression increased by 2.5-fold compared to the control group (t=3.06, P<0.05). Following 8 Gy irradiation, lipid expression levels were higher in the full-length PLIN5 overexpression group and lower in the PLIN5 knockdown group compared to wild-type cells. The truncated mutant lacking the lipid-binding domain showed no significant change in lipid content. Compared with the cell viability of (1.69±0.49)% in the empty vector irradiation group, the cell viability of the PLIN5 full-length overexpression group increased to (2.49±0.92)% (t=2.64, P<0.05). Compared with the cell viability of (2.54±0.80)% in the negative control exposure group, the cell viability of the PLIN5 interference group decreased to (1.89±0.45)% (t=3.76, P<0.05). No statistically significant difference was observed among the truncated mutant transfection group, wild-type cells, and the empty vector transfection group. Conclusions Under irradiation, up-regulated PLIN5 elevates lipid content in A549 cells, thereby enhancing their radioresistance and providing evidence that lipid-metabolic reprogramming is a critical determinant of tumor cell radioresistance. |
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