| Zhang Jiao,Wang Yongyi,Duan Min,et al.Impacts of eosinophil deficiency on acute radiation-induced lung injury[J].Chinese Journal of Radiological Medicine and Protection,2026,46(4):344-351 |
| Impacts of eosinophil deficiency on acute radiation-induced lung injury |
| Received:January 21, 2025 |
| DOI：10.3760/cma.j.cn112271-20250121-00029 |
| KeyWords:Eosinophil|γ-ray|Radiation-induced lung injury |
| FundProject: |
| Author Name | Affiliation | E-mail | | Zhang Jiao | College of Life Sciences, Hebei University, Baoding 071002, China
Academy of Military Medical Sciences, Academy of Military Science, Beijing 100850, China | | | Wang Yongyi | Academy of Military Medical Sciences, Academy of Military Science, Beijing 100850, China | | | Duan Min | Academy of Military Medical Sciences, Academy of Military Science, Beijing 100850, China | | | Yang Zhe | Academy of Military Medical Sciences, Academy of Military Science, Beijing 100850, China | | | Li Wencong | Academy of Military Medical Sciences, Academy of Military Science, Beijing 100850, China | | | Hao Yanhui | Academy of Military Medical Sciences, Academy of Military Science, Beijing 100850, China | | | Zuo Hongyan | Academy of Military Medical Sciences, Academy of Military Science, Beijing 100850, China | | | Li Yang | College of Life Sciences, Hebei University, Baoding 071002, China
Academy of Military Medical Sciences, Academy of Military Science, Beijing 100850, China | leeyoung109@hotmail.com |
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| Abstract:: |
| Objective To explore the impacts of eosinophil (EOS) deficiency on the progression of acute radiation-induced lung injury (RILI) Methods The Δdbl.GATA gene-edited mice were constructed by knocking out the high-affinity GATA binding site within the GATA-1 promoter region. The 8 to 10-week-old female and male Δdbl.GATA, as well as female and male wild-type (WT) C57BL/6J mice, 29 each type, were grouped into the control, 3 d post-irradiation, and 7 d post-irradiation groups using the random number table method. Mice in the latter two groups were exposed to total body irradiation at 8.5 Gy. At 3 d and 7 d post-irradiation, the lung indices of mice were calculated, the EOS-to-leukocyte ratios in the lung tissue of the mice were detected using flow cytometry, and the pulmonary function of the mice was evaluated using a small animal lung function testing system. Furthermore, the histopathological changes in the lung tissue were observed through hematoxylin and eosin (H&E) staining, and the TNF-α, IL-5, and IL-4 levels in the lung tissue were quantified by radioimmunoassay. Results Post-irradiation, compared to the mice in the control group, both female and male WT C57BL/6J gene-edited mice showed significantly lower EOS-to-leukocyte ratios of the lung tissue, with statistically significant differences (t =9.02-14.55, P < 0.001). Compared to the female WT C57BL/6J mice, the female Δdbl.GATA gene-edited mice exhibited significantly lower EOS-to-leukocyte ratios in the lung tissue (t =4.66, P < 0.01). Post-irradiation, both female and male WT C57BL/6J mice showed significantly lower relative peak inspiratory flow, relative peak expiratory flow, relative tidal volume, and relative minute ventilation volume than those before irradiation (t =3.30-9.20, P < 0.05). The above lung function parameters of the Δdbl.GATA gene-edited mice were significantly higher than those of the WT C57BL/6J mice (t = -5.59 to -2.61, P < 0.05). Additionally, the TNF-α, IL-5, and IL-4 levels of the lung tissue of the WT C57BL/6J mice were significantly higher than those of the control group (t = -4.06 to -2.68, P < 0.05), while these parameters in the lung tissue of the female Δdbl.GATA gene-edited mice were significantly lower than those in the female WT C57BL/6J mice (t =2.12-3.23, P < 0.05). No significant differences were observed in the lung index and histopathological changes of lung tissue between Δdbl.GATA gene-edited and WT C57BL/6J mice. Conclusion EOS deficiency can alleviate radiation-induced lung injury in mice. |
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