| Wang Mimi,Tian Xuelei,Liu Qingjie.Screening of radiation-responsive lncRNAs and their preliminary validation as tissue damage biomarkers in a whole-body irradiated mouse model[J].Chinese Journal of Radiological Medicine and Protection,2026,46(3):238-245 |
| Screening of radiation-responsive lncRNAs and their preliminary validation as tissue damage biomarkers in a whole-body irradiated mouse model |
| Received:October 20, 2025 |
| DOI：10.3760/cma.j.cn112271-20251020-00366 |
| KeyWords:LncRNAs Radiation-induced tissue injury Ionizing radiation Biomarker |
| FundProject:国家自然科学基金(82173463) |
| Author Name | Affiliation | E-mail | | Wang Mimi | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Xuelei | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | liuqingjie@nirp.chinacdc.cn |
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| Abstract:: |
| Objective To explore the potential of long non-coding RNAs (lncRNAs) as tissue-specific or universal radiation biomarkers in a mouse model following whole-body irradiation. Methods A preliminary screening was conducted to identify lncRNAs that are homologous between humans and mice and known to be induced by radiation, utilizing public databases from both domestic and international sources. Mice were exposed to a single dose of whole-body irradiation using 60Co γ-rays at 0, 2, 4 and 6 Gy. At 48 h post-irradiation, multiple tissues, plasma and blood cells of irradiated mice were collected. The expression levels of candidate lncRNAs across these samples, along with their dose-dependent changes, were quantified by real-time quantitative PCR. Results A total of 19 lncRNAs that are homologous in both humans and mice and known to be radiation-induced were screened out through literature retrieval. Real-time quantitative PCR assay showed that Dino, Trp53cor1 and Pvt1 were significantly up-regulated in multiple tissues after irradiation (t = 3.69-10.11, 2.56-11.74, 2.22-12.98, P< 0.05). Notably, Dino and Trp53cor1 exhibited a clear dose-dependent response (R2 = 0.92-0.99, 0.82-0.99, P< 0.05), and they showed consistent up-regulation patterns in plasma and blood cells (t = 2.46-5.54, 5.02-7.14, R2 = 0.70-0.99, 0.63-0.78, P < 0.05). The expression of Pvt1 was significantly up-regulated at 2 Gy and 4 Gy in plasma (t = 3.11, 3.83, P < 0.05), whereas it exhibited a dose-dependent down-regulation in blood cells (t = -13.77 to -9.89, R2 = 0.86, P< 0.05). After irradiation, the expression of Tmevpg1 was significantly downregulated only in lung and colon (t = -8.03 to -2.61, P < 0.05), and exhibited a favorable dose-dependent change in lung tissue (R2 = 0.76, P < 0.05), indicating certain tissue-specific characteristics. Moreover, its down-regulation trend in plasma and blood cells was consistent with the tissue level (Z = -2.88 to -2.42, t = -11.12 to -8.32, R2 = 0.81, 0.74, P<0.05). Conclusions The changes in the expression levels of Dino and Trp53cor1 have the potential to serve as universal biomarkers for radiation exposure. The change in the expression level of Tmevpg1 suggests that it has the potential to serve as an early, minimally invasive warning marker for radiation damage to the lungs and large intestine. |
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