| Yan Juan,Cai Tianjing,Lu Xue,et al.Differential biological effects of FLASH irradiation on BEAS-2B and A549 cells[J].Chinese Journal of Radiological Medicine and Protection,2026,46(1):13-19 |
| Differential biological effects of FLASH irradiation on BEAS-2B and A549 cells |
| Received:August 18, 2025 |
| DOI：10.3760/cma.j.cn112271-20250818-00301 |
| KeyWords:Ultra-high dose rate Oxidative damage DNA double-strand breaks Cell cycle |
| FundProject:国家自然科学基金(12175114);中国疾病预防控制中心辐射防护与核安全医学所青年科学研究所长基金(2021-01) |
| Author Name | Affiliation | E-mail | | Yan Juan | Key Laboratory of Radiological Protection and Nuclear Emergency, ChinaCDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Cai Tianjing | Key Laboratory of Radiological Protection and Nuclear Emergency, ChinaCDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, ChinaCDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | liuqingjie@nirp.chinacdc.cn | | Li Shuang | Key Laboratory of Radiological Protection and Nuclear Emergency, ChinaCDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | Key Laboratory of Radiological Protection and Nuclear Emergency, ChinaCDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | |
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| Abstract:: |
| Objective To compare the differential effects of FLASH and conventional dose rate (CONV, 0.3 Gy/s) electron beam irradiation on normal and tumor cells. Methods Using a 6 MeV electron beam linear accelerator, BEAS-2B and A549 were irradiated separately to establish a control group, CONV group, and FLASH group. Using CCK8 experiment to detect the effects of different dose rates of irradiation on the survival ability of normal cells and tumor cells. The oxidative damage was determined by measuring the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in cells after irradiation; Immunofluorescence assay was used to detect double stranded DNA breaks and repair in cells, while flow cytometry was used for cell cycle detection. Results After irradiation with CONV and FLASH at 6 and 15 Gy, the survival rate of BEAS-2B cells in the FLASH group was significantly increased compared to the CONV group (t = 5.17-7.58, P<0.05). And as a normal cell, the survival rate of BEAS-2B is higher than that of A549 cells (t = 3.63-7.79, P<0.05). After CONV irradiation, the SOD activity (tBEAS-2B = 2.90-10.39, P<0.05; tA549 = 8.96-10.76, P<0.05) and MDA content (tBEAS-2B = 8.69-11.94, P<0.05; tA549 = 10.44-14.08, P<0.05) of BEAS-2B and A549 cells were significantly altered. After FLASH irradiation, there was little change in SOD activity in BEAS-2B cells, and the difference was not statistically significant compared with CON (P>0.05). There was no statistically significant difference in SOD activity between the FLASH group and the CONV group in A549 cells (P>0.05). The MDA content of BEAS-2B cells in FLASH group was lower than that in CONV group (t = 6.21-10.12, P<0.05); Compared with the CONV group, there was no significant difference in MDA content in A549 after FLASH irradiation. Compared with CONV, after FLASH irradiation, BEAS-2B cells showed stable G2 phase arrest at 24-72 h, while A549 cells showed a high proportion of G1 phase arrest at 4 h, but cell cycle collapsed at 72 h. At 4 h after irradiation, the FLASH group showed less formation of gamma H2AX foci in BEAS-2B cells compared to the CONV group, and the γ-H2AX foci significantly decreased after 24 h. After FLASH irradiation, in DNA double-strand breaks was reduced the number, and then were repaired more quickly. Conclusions After FLASH irradiation, the radiation damage to lung epithelial-like cells BEAS-2B in terms of survival, oxidative damage, cell cycle, and DNA damage repair is less than that of CONV irradiation, the radiation damage of A549 cells after FLASH irradiation is bascally consistent with that after CONV irradiation. |
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