| Cui Zhilin,Yang Meiying,Chang Chenxu,et al.Effect of electromagnetic field exposure on the structure and function of the primary auditory cortex in mice[J].Chinese Journal of Radiological Medicine and Protection,2026,46(1):5-12 |
| Effect of electromagnetic field exposure on the structure and function of the primary auditory cortex in mice |
| Received:November 21, 2024 |
| DOI：10.3760/cma.j.cn112271-20241121-00451 |
| KeyWords:Electromagnetic field Composite exposure Primary auditory cortex Auditory electrophysiological Prepulse inhibition |
| FundProject: |
| Author Name | Affiliation | E-mail | | Cui Zhilin | College of Basic Medicine, Chengde Medical College, Chengde 067000, China
Academy of Militray Medical Sciences, Beijing 100850, China | | | Yang Meiying | Academy of Militray Medical Sciences, Beijing 100850, China | | | Chang Chenxu | Academy of Militray Medical Sciences, Beijing 100850, China | | | Shi Lei | Academy of Militray Medical Sciences, Beijing 100850, China | | | Wang Yue | Academy of Militray Medical Sciences, Beijing 100850, China | | | Lu Chunxue | Academy of Militray Medical Sciences, Beijing 100850, China | | | Liu Ying | Academy of Militray Medical Sciences, Beijing 100850, China | | | Hao Yanhui | Academy of Militray Medical Sciences, Beijing 100850, China | zuohy2005@126.com | | Li Yang | Academy of Militray Medical Sciences, Beijing 100850, China | | | Zuo Hongyan | Academy of Militray Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore the effect of combined exposure to static magnetic field (SMF) and microwave radiation on the structure and function of the primary auditory cortex (Au1) in mice, and to provide new insights into the mechanisms underlying biological effects and protection strategies for composite electromagnetic field (EMF) exposure. Methods C57BL/6N mice were randomly divided divided into four groups: control group, SMF exposure group, microwave exposure group, and a combined EMF expsoure group. The combined exposure group was exposed to a 100 mT SMF for 1 h, followed by microwave radiation with a center frequency of 9.875 GHz and SAR value of 2.58 W/kg for 15 min. The SME group received only the above SME exposure, the microwave group only received the above microwave radiation, and the control group underwent sham expsoure under identical conditions. The prepulse inhibition (PPI) of startle reflex test was used to evaluate auditory neurobehavioral changes induced by composite EMF exposure. Auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) were employed to acess alterlations in auditory electrophysiological function. Light microscopy and transmission electron microscopy (TEM) were utilized to observe morphological and ultrastructural changes in the PAC. Results At 3, 14 d post-exposure, the PPI percentage in the combined exposure group was significantly higher than that before exposure and in the single SMF or microwave exposure groups (P＜0.05). At 7, 14, and 28 d post-exposure, the latency of ABR wave I was significantly prolonged compared with the control group and the single exposure groups (P＜0.05), while the DPOAE amplitude was significantly reduced (P＜0.05), with no significant change in ABR threshold. Morphological and ultrastructural observations revealed neuronal degeneration and apoptosis, blurred synaptic clefts, and swelling of glial mitochondria in the Au1, with the most prominent changes detected in the combined exposure group at 7 d post-exposure. Conclusions Combined exposure to SMF and microwave radiation impairs the function of cochlear outer hair cells, inhibits the upward conduction function of the cochlear nerve pathway, induces histopathological changes in the Au1, and disrupts sensorimotor gating function, but exerts no effect on hearing threshold. |
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