| Zhang Jun,Zhang Siqian,Wang Qiaojuan,et al.Effects of proton FLASH irradiation and conventional irradiation on the cell cycle and death of renal cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2025,45(11):1100-1106 |
| Effects of proton FLASH irradiation and conventional irradiation on the cell cycle and death of renal cancer cells |
| Received:July 01, 2025 |
| DOI：10.3760/cma.j.cn112271-20250701-00217 |
| KeyWords:FLASH radiation Conventional radiation Cell cycle Apoptosis Pyroptosis |
| FundProject:国家自然科学基金面上项目(12575369,12275192);中核集团青年英才项目;学科建设托举工程项目(XKTJ-XK202410) |
| Author Name | Affiliation | E-mail | | Zhang Jun | Department of Pathology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Zhang Siqian | Department of Pathology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Wang Qiaojuan | China Institute of Atomic Energy, Beijing 102413, China | | | Sui Li | China Institute of Atomic Energy, Beijing 102413, China | | | Zhang Yongsheng | Department of Pathology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Cao Zhifei | Department of Pathology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | hunancao@163.com |
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| Abstract:: |
| Objective To explore the effects of proton FLASH irradiation (FLASH-IR) and conventional irradiation (CONV-IR) on the cell cycle, apoptosis, and pyroptosis of renal cancer cells. Methods Renal cancer cells (769-P) were irradiated with 8 Gy of protons at a dose rate of 40 Gy/s for FLASH-IR and 0.4 Gy/s for CONV-IR, Ctrl group was treated without irradiation. Cells were collected 24 h after irradiation. The changes in the cell cycle were measured using flow cytometry. The expression of genes and proteins related to the cell cycle, apoptosis, and pyroptosis signaling pathways in renal cancer cells was measured using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blot. Results Proton FLASH-IR increased the proportion of renal cancer cells in the G0/G1 phase [FLASH-IR group vs. Ctrl group, (67.01±0.44)% vs. (38.68±0.63)%, t = -63.99, P＜0.05], while CONV-IR increased the proportion of renal cancer cells in the G2/M phase [CONV-IR group vs. Ctrl group, (56.65±1.52)% vs. (23.67±0.51)%, t = -29.17, P＜0.05]. Both proton FLASH-IR and CONV-IR caused apoptosis of renal cancer cells (tFLASH= -16.24 to -5.01, P <0.05; tCONV=-20.08 to 6.11, P< 0.05) and CONV-IR activated the P53/P21 pathway (t = -16.86 to -9.74, P < 0.05). Both proton FLASH-IR and CONV-IR induced pyroptosis of renal cancer cells (tFLASH= -23.36 to 20.18, P <0.05; tCONV=-41.62 to 13.95, P<0.05), and the former exhibited a greater effect (FLASH-IR group vs. CONV-IR group, 0.96±0.01 vs. 0.68±0.44, t = -10.46, P <0.05). Conclusions Both proton FLASH-IR and CONV-IR bring about changes in the cell cycle of renal cancer, promoting apoptosis and pyroptosis. However, there are differences between the two mechanisms that require further exploration. Proton FLASH-IR holds promise for the treatment of renal cancer. |
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