| Wang Yuanxu,Li Jinhua,Tang Yufeng.Effects of X-ray FLASH irradiation and conventional-dose-rate irradiation on radiation-induced injury to the brain tissue of mice[J].Chinese Journal of Radiological Medicine and Protection,2025,45(11):1069-1076 |
| Effects of X-ray FLASH irradiation and conventional-dose-rate irradiation on radiation-induced injury to the brain tissue of mice |
| Received:July 10, 2025 |
| DOI：10.3760/cma.j.cn112271-20250710-00244 |
| KeyWords:FLASH irradiation Ultra-high dose rate Cognitive function |
| FundProject: |
| Author Name | Affiliation | E-mail | | Wang Yuanxu | School of Medicine, University of Electronic Science and Technology of China, Chengdu 610000, China | | | Li Jinhua | Department of Neurology, Mianyang Central Hospital, Mianyang 621000, China | | | Tang Yufeng | School of Medicine, University of Electronic Science and Technology of China, Chengdu 610000, China
Department of Neurology, Mianyang Central Hospital, Mianyang 621000, China
Sichuan Provincial Engineering Research Center of Nuclear Medical Equipment Translation and Application, Mianyang Central Hospital, Mianyang 621000, China | dryufeng@126.com |
|
| Hits: 3393 |
| Download times: 540 |
| Abstract:: |
| Objective To evaluate the effects of FLASH irradiation(FLASH-RT) and conventional irradiation (CONV-RT) on radiation-induced injury to the brain tissue of healthy mice using a domestically developed X-FLASH platform. Methods Ninety healthy 8-week-old male C57BL/6J mice were randomly divided into sham irradiation(Sham) group, CONV-RT group, and FLASH-RT group, with 30 mice in each group. A single dose of 20 Gy whole-brain radiotherapy was administered, with a dose rate of 339 Gy/s for FLASH radiotherapy and 0.067 Gy/s for conventional radiotherapy. The effects of different radiotherapy modalities on neurological function and neuroinflammation were assessed using water maze test, open field test, Nissl staining, and immunohistochemical analysis (GFAP, Iba-1). Results There were significant differences in body weight between the CONV group and the FLASH group at 8, 9, 10, 11, 12, and 24 d after radiotherapy (q=2.82-5.31, P<0.05). At 4 months after radiotherapy, there were significant differences between the FLASH group and the CONV group in escape latency, platform crossings, central zone crossings, and Thigmotaxis distance ratio (q=3.72, 2.92, 3.28, 2.97, P<0.05). Quantitative Nissl staining result revealed significant differences between the CONV group and the FLASH group in the CA3 and DG regions (q=3.61, 3.21, P<0.05), but not in the CA1 region (P>0.05). Immunohistochemical result showed a significant difference between the CONV group and the FLASH group in the percentage of GFAP+ positive area (q=2.28, P<0.05), but not in the number of Iba-1+ cells (P>0.05). Conclusions Compared to conventional radiotherapy, X-FLASH radiotherapy causes less radiation-induced injury to the brain tissue, thus holding a promising prospect for clinical translation. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
