| Zhang Tianxi,Ye Haipeng,Yu Huijuan,et al.Establishing a dose-response curve for 137Cs γ-ray irradiation-induced chromosomal aberrations in human peripheral blood lymphocytes through automated analysis[J].Chinese Journal of Radiological Medicine and Protection,2025,45(9):858-862 |
| Establishing a dose-response curve for 137Cs γ-ray irradiation-induced chromosomal aberrations in human peripheral blood lymphocytes through automated analysis |
| Received:May 14, 2025 |
| DOI：10.3760/cma.j.cn112271-20250514-00169 |
| KeyWords:Automated analysis Chromosomal aberration Dose-response curve γ-rays Unstable chromosome aberration |
| FundProject:杭州市医药卫生科技项目(A20230012);浙江省卫生信息学会科研计划项目(2024XHZN-Y02) |
| Author Name | Affiliation | E-mail | | Zhang Tianxi | Hangzhou Hospital of Prevention and Treatment of Occupation Diseases, Hangzhou 310014, China | | | Ye Haipeng | Hangzhou Hospital of Prevention and Treatment of Occupation Diseases, Hangzhou 310014, China | | | Yu Huijuan | Hangzhou Hospital of Prevention and Treatment of Occupation Diseases, Hangzhou 310014, China | | | Liu Yan | Hangzhou Hospital of Prevention and Treatment of Occupation Diseases, Hangzhou 310014, China | | | Bi Yaping | Hangzhou Hospital of Prevention and Treatment of Occupation Diseases, Hangzhou 310014, China | | | Le Yanna | Hangzhou Hospital of Prevention and Treatment of Occupation Diseases, Hangzhou 310014, China | leyanna612@163.com |
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| Abstract:: |
| Objective To establish a dose-response curve of dicentric chromosomes and centromeric rings (dic+r) in γ-ray irradiation-induced chromosomal aberrations in human peripheral blood lymphocytes through automated analysis. Methods Peripheral blood samples from three healthy donors were irradiated in vitro at doses of 0,0.5,0.75,1,1.5,2,3,4, and 5 Gy and a dose rate of 0.80 Gy/min using a 137Cs γ-ray source. Post-irradiation, lymphocytes were cultured based on standard protocols, harvested using an automatic cell harvester, and prepared on slides using an automatic slide preparation system. dic+r were analyzed fully automatically using the DCScore software, and a dose-response curve of dic+r was established through fitting and then validated using the CABAS software. Results The dose-response curve followed a linear-quadratic model, i.e., y = 0.093 65+0.030 21D+ 0.025 31D2 (R2 = 0.999 2), where y was the quantity of dic+r and D was the absorbed dose of γ-ray irradiation (Gy). Doses to samples for blind validation were estimated using this curve, yielding deviations of less than 24% from the actual irradiation doses. Conclusions The fully automated analysis of dic+r in 137Cs γ-ray irradiation-induced chromosomal aberrations, followed by the construction of the dose-response curve, holds significant potential for rapid, high-throughput biodosimetry in large-scale nuclear emergencies. |
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