| Li Xueda,Tian Chuan,Wang Wenfei,et al.Application of a 3D radioactive seed model to glioma cells in mice[J].Chinese Journal of Radiological Medicine and Protection,2025,45(6):544-550 |
| Application of a 3D radioactive seed model to glioma cells in mice |
| Received:August 23, 2024 |
| DOI：10.3760/cma.j.cn112271-20240823-00317 |
| KeyWords:3D printing Model 125I radioactive seed |
| FundProject:国家重点研发计划中美政府间合作项目(2019DEY120100) |
| Author Name | Affiliation | E-mail | | Li Xueda | Department of the Interventional Medical Center, Affiliated Hospital of Qingdao University, Qingdao 266000, China | | | Tian Chuan | Department of the Interventional Medical Center, Affiliated Hospital of Qingdao University, Qingdao 266000, China | | | Wang Wenfei | Department of Radiology, Shengli Oilfield Central Hospital, Dongying 257034, China | | | Jiang Peng | Department of Radiology, Affiliated Hospital of Qingdao University, Qingdao 266000, China | | | Zhang Hongtao | Department of Oncology, Hebei Provincial People's Hospital, Shijiazhuang 050051, China | | | Wang Congxiao | Department of the Interventional Medical Center, Affiliated Hospital of Qingdao University, Qingdao 266000, China | | | Liu Shifeng | Department of the Interventional Medical Center, Affiliated Hospital of Qingdao University, Qingdao 266000, China | | | Hu Xiaokun | Department of the Interventional Medical Center, Affiliated Hospital of Qingdao University, Qingdao 266000, China | huxiaokun770@163.com | | Li Teng | School of Electrical Engineering and Automation, Anhui University, Hefei 230039, China | |
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| Abstract:: |
| Objective To design a novel model for experiments on in vitro irradiation with radioactive seeds using a treatment planning system (TPS) and 3D printing technology and to preliminarily validate the design scientific rigor of the model via experiments on isodose brachytherapy (BT) and external beam radiotherapy (EBRT) on glioma cells in mice. Methods The TPS was employed to design the model's shape and calculate the number and positions of radioactive seeds, and 3D printing technology was utilized to fabricate the experimental model. The GL261 cell line was selected for in vitro irradiation experiments, with the mice divided into the control, EBRT, and BT groups. Mice in the EBRT and BT groups were treated with EBRT and BT, respectively, at doses of 2, 4, and 6 Gy. Then, changes in their cell viability, proliferation, and the level of intracellular reactive oxygen species (ROS) were assessed. Results The model for in vitro irradiation with radioactive seeds was successfully designed and fabricated. The single photon emission computed tomography (SPECT) verified a uniform radioactive distribution within the model, with no significant cold spots. The BT and EBRT groups displayed decreased cell viability with an increase in the radiation dose. Compared to the EBRT group, the BT group exhibited significantly reduced cell viability (51.33% vs. 22.00%, t= 10.94, P< 0.05) and clone counts (172.67 ± 13.11 vs. 53.67 ± 10.22, t = 8.73, P< 0.05), but a significantly increased level of ROS (102.52 ± 6.8 vs. 144.81 ± 6.01, t = -5.26, P< 0.05) at a dose of 6 Gy. Conclusions An effective model of in vitro irradiation with radioactive seeds is designed based on TPS and 3D printing technology. This provides an experimental model tool and target for research on the BT and EBRT mechanisms. |
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