| Wang Yalin,Li Shuang,Sun Xin,et al.Screening and preliminary validation of differentially expressed lncRNAs in human lymphocytes induced by low dose ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2025,45(5):423-430 |
| Screening and preliminary validation of differentially expressed lncRNAs in human lymphocytes induced by low dose ionizing radiation |
| Received:January 06, 2025 |
| DOI：10.3760/cma.j.cn112271-20250106-00009 |
| KeyWords:Ionizing radiation Low dose Long non-coding ribonucleic acid (lncRNA) Biomarker |
| FundProject:国家自然科学基金(82173464,82173463) |
| Author Name | Affiliation | E-mail | | Wang Yalin | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Diease Control and Protection, Beijing 100088, China | | | Li Shuang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Diease Control and Protection, Beijing 100088, China | | | Sun Xin | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Diease Control and Protection, Beijing 100088, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Diease Control and Protection, Beijing 100088, China | | | Cai Tianjing | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Diease Control and Protection, Beijing 100088, China | | | Liu Qingjie | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Diease Control and Protection, Beijing 100088, China | liuqingjie@nirp.chinacdc.cn |
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| Abstract:: |
| Objective To investigate the changes in the expression levels of long non-coding ribonucleic acids (lncRNAs) in human lymphocytes induced by low-dose ionizing radiation (LDIR) and the potential of lncRNAs as radiation biomarkers. Methods Human immortalized lymphocytes (AHH-1) were irradiated with 0, 0.05, and 0.1 Gy of γ-rays at 24 h to extract RNAs for whole transcriptome sequencing. The sequencing was performed based on the 0, 0.05, and 0.1 Gy groups. The differentially expressed lncRNAs induced by LDIR were identified. The molecular functions, biological processes, and signaling pathway enrichment of differentially expressed genes were analyzed through the Gene Ontology (GO) analysis. Candidate lncRNAs were preliminarily validated using the qRT-PCR method. AHH-1 cells were irradiated with 0, 0.02, 0.05, 0.075, 0.1, and 0.2 Gy to extract the total RNAs at 4, 24, 48, 72, 96, and 120 h. The dose-response relationship of candidate lncRNAs was detected and analyzed. Peripheral blood sampled from eight healthy persons was irradiated with 0, 0.02, 0.05, 0.075, 0.1, and 0.2 Gy in vitro, followed by culturing for 24 h and 48 h to further verify the changes in the expression levels of radiation-responsive lncRNAs at the cellular level. Results A total of 44 lncRNAs that were significantly up- or down-regulated after 0.05 and 0.1 Gy irradiation were initially identified through transcriptome sequencing. Among them, lncRNAs with over two-fold differential expression included SNHG1, SNHG15, NEAT1, and PRC1-AS1. At the cellular level, compared to 0 Gy, the relative expression level of PRC1-AS1 after 4 h to 48 h of γ-ray irradiation, was significantly elevated at 0.05, 0.075, and 0.1 Gy(t= -3.11 to 1.23, P < 0.05). In contrast, the relative expression level of NEAT1 was significantly up-regulated in a dose range of 0.02 to 0.1 Gy (t=-2.47 to 2.10, P < 0.05). At the level of human peripheral blood, the relative expression levels of PRC1-AS1 and NEAT1 were significantly increased at 24 h after 0 to 0.2 Gy irradiation (t=-3.79 to -1.96, P < 0.05). Conclusion The PRC1-AS1 and NEAT1 with significant changes in expression levels serve as potential LDIR biomarkers. |
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