| Wu Zhuojun,Huang Zhifei,Li Jiangyi,et al.Impacts of ionizing radiation dose on NADPH oxidase expression in mouse intestine[J].Chinese Journal of Radiological Medicine and Protection,2025,45(5):399-407 |
| Impacts of ionizing radiation dose on NADPH oxidase expression in mouse intestine |
| Received:October 30, 2024 |
| DOI：10.3760/cma.j.cn112271-20241030-00417 |
| KeyWords:Ionizing radiation Dual oxidase 2 (DUOX2) Intestine Nicotinamide adenine dinucleotide phosphate (NADPH) |
| FundProject: |
| Author Name | Affiliation | E-mail | | Wu Zhuojun | Fourth Military Medical University, State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Xi'an 710032, China | | | Huang Zhifei | Radiation Protection Medicine, Department of Preventice Medicine, Air Force Medical University Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an 710032, China | | | Li Jiangyi | Radiation Protection Medicine, Department of Preventice Medicine, Air Force Medical University Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an 710032, China | | | Wang Xing | Radiation Protection Medicine, Department of Preventice Medicine, Air Force Medical University Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an 710032, China | | | Guo ling | Radiation Protection Medicine, Department of Preventice Medicine, Air Force Medical University Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an 710032, China | | | Ding Guirong | Radiation Protection Medicine, Department of Preventice Medicine, Air Force Medical University Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an 710032, China | dingzhao@fmmu.edu.cn |
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| Abstract:: |
| Objective To investigate the effects of different doses of ionizing radiation on the changes in nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and reactive oxygen species (ROS) levels in the intestines of mice. Methods C57BL/6 mice aged 6-8 weeks were randomly assigned to four groups (0 Gy, 0.1 Gy, 0.2 Gy, and 0.5 Gy; n=10/group) and subjected to single whole-body irradiation using a 60Co γ-ray source at a dose rate of 13 mGy/min. At 20 weeks post-irradiation, jejunal, ileal, and colonic tissues were collected. Immunohistochemistry, Western blotting, and reverse transcription-polymerase chain reaction (RT-PCR) were employed to assess mRNA and protein expression of NADPH oxidase components. Hydrogen peroxide (H2O2) levels and the expression of Nuclear Factor-kappa B (NF-κB), a transcriptional regulator of Dual Oxidase 2 (DUOX2), were also measured. Results Compared with the 0 Gy group, mice in the 0.5 Gy group exhibited shortened villus length in the jejunum, villus fusion in the ileum, and increased crypt spacing in the colon, with statistically significant differences (t=2.48, P < 0.05). No significant differences were observed in other dose groups compared to the 0 Gy group (P > 0.05).The expression of H2O2 in the jejunum, ileum, and colon of the 0.1 Gy group was significantly elevated compared to the 0 Gy group (t=4.12, 3.12, 3.12; P < 0.05). In the 0.5 Gy group, H2O2 expression in the jejunum and colon increased nearly twofold relative to the 0 Gy group (t=8.67, 8.69; P < 0.05).At 20 weeks post-irradiation, DUOX2 protein expression levels in the jejunum, ileum, and colon were markedly higher in irradiated mice than in the 0 Gy group (t=3.03, 10.29, 2.74; P < 0.05). DUOX2 mRNA levels in the jejunum, ileum, and colon of the 0.1 Gy group were significantly upregulated compared to the 0 Gy group (t=12.75, 4.12, 11.14; P < 0.05). Additionally, NOX4 mRNA expression increased in the jejunum of the 0.2 Gy group (t=4.54, P < 0.05) and in the ileum of the 0.1 Gy group (t=4.13, P < 0.05).The nuclear factor kappa-B (NF-κB), a transcriptional regulator of DUOX2, showed an upward trend in expression in the jejunum, ileum, and colon of the 0.1 Gy group compared to the 0 Gy group, with statistically significant differences (t=8.73, 8.18, 7.02; P < 0.05). Conclusion Low-dose radiation induces long-term effects on the intestinal tract. Specifically, 0.5 Gy irradiation causes mild morphological alterations in the jejunum, ileum, and colon, while 0.1 Gy irradiation promotes the upregulation of DUOX2, a NADPH oxidase, in intestinal tissues. |
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