| Li Huixian,Chen Huifeng,Huang Weixu,et al.Transcriptomic analysis of differentially expressed mRNAs in the peripheral blood of residents in the Yangjiang high-background radiation area[J].Chinese Journal of Radiological Medicine and Protection,2025,45(5):392-398 |
| Transcriptomic analysis of differentially expressed mRNAs in the peripheral blood of residents in the Yangjiang high-background radiation area |
| Received:July 19, 2024 |
| DOI：10.3760/cma.j.cn112271-20240719-00269 |
| KeyWords:High background radiation area Low-dose ionizing radiation Transcriptomics Mitophagy Aging |
| FundProject:广东省基础与应用基础研究基金(2019A1515011969,2022A1515012421,2023A1515010414,2025A1515012483);广州市科技计划项目(202002030031) |
| Author Name | Affiliation | E-mail | | Li Huixian | School of Public Health, Sun Yat-sen University, Guangzhou 510080, China
Institute of Radiation Hygiene and Protection, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Chen Huifeng | Institute of Radiation Hygiene and Protection, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Huang Weixu | Institute of Radiation Hygiene and Protection, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Chen Guanyou | School of Public Health, Sun Yat-sen University, Guangzhou 510080, China | | | Zhang Min | Institute of Radiation Hygiene and Protection, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Li Daochuan | School of Public Health, Sun Yat-sen University, Guangzhou 510080, China | | | Zou Jianming | School of Public Health, Sun Yat-sen University, Guangzhou 510080, China
Institute of Radiation Hygiene and Protection, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | zjm990916@126.com |
|
| Hits: 931 |
| Download times: 466 |
| Abstract:: |
| Objective To investigate differential gene expression, enriched biological processes, and pathway differences between residents of a high-background radiation area (HBRA), Yangjiang, and a control area—Enping. Methods Seven residents were selected from the HBRA (the HBRA group) and seven from the control area (the control group) using the two-stage random sampling method. The cumulative radiation dose for each individual was calculated based on the ambient gamma exposure levels. Peripheral blood samples were collected and analyzed via high-throughput sequencing to identify differentially expressed genes. Subsequent analyses included gene ontology (GO) for biological process (BP), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and gene set enrichment analysis (GSEA). Results The median ages of the HBRA and control groups had no statistically significant difference (P = 0.370). The cumulative external doses for the HBRA and control groups were (99.59±20.07) and (33.82±10.61) mSv, respectively. This difference was statistically significant (t = -5.88, P = 0.001). High-throughput sequencing identified 1 224 differentially expressed genes in the HBRA group, including 32 senescence-related genes, compared to the control group. The result of GO-BP analysis showed that these genes were predominantly enriched in cell signaling, biosynthesis, localization, cell cycle regulation, and cellular stress responses. KEGG analysis revealed significant enrichment in the chemokine and MAPK signaling pathways, as well as in pathways related to the cell cycle, autophagy, and mitophagy. Furthermore, GSEA analysis confirmed that the differentially expressed genes were mainly related to cell cycle regulation and mitochondrial functions. Conclusions Differentially expressed mRNAs are found in the peripheral blood of residents in the HBRA. These mRNAs are predominantly associated with key biological processes and pathways, including cell cycle regulation, chemokine and MAPK signaling pathways, and mitophagy. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
