| Yi Ruhan,Lu Xue,Cai Tianjing,et al.Study on the role of Caveolin-1 in ionizing radiation-induced premature senescence of vascular endothelial cells[J].Chinese Journal of Radiological Medicine and Protection,2025,45(3):163-169 |
| Study on the role of Caveolin-1 in ionizing radiation-induced premature senescence of vascular endothelial cells |
| Received:November 14, 2024 |
| DOI：10.3760/cma.j.cn112271-20241114-00439 |
| KeyWords:Irradiation X-rays Vascular endothelial cells Premature senescence Caveolin-1 |
| FundProject: |
| Author Name | Affiliation | E-mail | | Yi Ruhan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Cai Tianjing | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Gao Ling | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | gaoling@nirp.chinacdc.cn |
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| Abstract:: |
| Objective To explore the role of Caveolin-1 (CAV-1) in radiation-induced premature senescence of vascular endothelial cells. Methods A cell model with stable knockdown of CAV-1 was constructed in human microvascular endothelial cells (HMEC-1) by lentiviral transfection using puromycin screening. The cells were divided into NC group and sh-CAV-1 group based on whether they were infected with lentivirus shRNA-CAV-1. The protein expression levels of CAV-1, p53 and p21 were detected by Western blot at 24, 48, and 72 h after 0, 2, and 4 Gy X-ray irradiation. The β-galactosidase staining kit was used to detect β-galactosidase in cells. CCK-8 kit was used to detect cell viability, and vascular endothelial cell function was detected by vascular tube-forming assay. Results CAV-1 protein expression was significantly decreased at 48 h after 2 and 4 Gy X-ray irradiation (t= 3.50, 3.89, P < 0.05), and β-galactosidase in sh-CAV-1 group was significantly increased at 72 h after 0, 2 and 4 Gy X-ray irradiation (t= 12.91, 11.54, 6.04, P< 0.05) compared with the NC group. Knockdown of CAV-1 resulted in the decrease in the expression level of the cellular senescence-associated protein p53 protein (t= 4.09, 3.13, 3.43, P< 0.05), but increase in the expression level of p21 protein (t= -3.63, -3.33, -3.06, P< 0.05). Compared with the NC group, knockdown CAV-1 significantly decreased cell viability (t= 2.97-25.89, P< 0.05) and reduced vessel-forming capacity (t= 3.39-39.68, P< 0.05). Conclusions CAV-1 is involved in the process of radiation-induced premature senescence of vascular endothelial cells through positive regulation of p53 and negative regulation of p21. |
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