| Wu Shuang,Li Zhihui,Yang Wenxi,et al.Effects of pulsed electron beams with ultra-high dose rate and conventional dose rate on Caenorhabditis elegans[J].Chinese Journal of Radiological Medicine and Protection,2024,44(11):903-908 |
| Effects of pulsed electron beams with ultra-high dose rate and conventional dose rate on Caenorhabditis elegans |
| Received:March 20, 2024 |
| DOI：10.3760/cma.j.cn112271-20240320-00096 |
| KeyWords:Ultra-high dose rate Electron beams Caenorhabditis elegans Development Life span |
| FundProject: |
| Author Name | Affiliation | E-mail | | Wu Shuang | Hengyang Medical School, Graduate Collaborative Training Base of Academy of Military Sciences, University of South China, Hengyang 421001, China
Institute of Radiation Medicine, Academy of Military Medical Science, Academy of Military Science, Beijing 100850, China | | | Li Zhihui | Institute of Radiation Medicine, Academy of Military Medical Science, Academy of Military Science, Beijing 100850, China | | | Yang Wenxi | Institute of Radiation Medicine, Academy of Military Medical Science, Academy of Military Science, Beijing 100850, China | | | Dong Guofu | Institute of Radiation Medicine, Academy of Military Medical Science, Academy of Military Science, Beijing 100850, China | | | Wang Changzhen | Institute of Radiation Medicine, Academy of Military Medical Science, Academy of Military Science, Beijing 100850, China | wangcz2002@aliyun.com |
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| Abstract:: |
| Objective To explore the effects of ultra-high dose rate pulsed electron beams on Caenorhabditis elegans (C. elegans). Methods The adult wild-type strain (N2) of C. elegans was synchronized and cultured to L4 stage, and then randomly divided into control group (SHAM group), conventional radiotherapy dose rate group (CONV group) and ultra-high dose rate radiation group (UHDR group). The CONV and UHDR groups were exposed to 3 Gy of the pulsed electron beam at dose rates of 0.3 and 200 Gy/s, respectively. After irradiation, the egg-laying capacity of each group was assessed, and the developmental progress, motility, and survival rates each were evaluated at day 3, 6, and 10. Results On the 3rd day post-irradiation, both the CONV and UHDR groups showed shorter body lengths compared to the SHAM group (t=4.81, 4.83, P<0.05), with no significant differences in body width (P>0.05). On the 6th and 10th days, the CONV group showed a significant reduction in both body length and width compared to the SHAM group (t=3.18-3.63, P<0.05), whereas the UHDR group displayed a significant increase in body length (t=-9.85, -2.87, P<0.05) with no significant change in body width. When comparing the UHDR group to the CONV group on day 6 and 10, a significant increase in body width was observed (t=-4.43, -3.37,P<0.05). Motor activity, including head swinging and body bending, significantly decreased in the CONV and UHDR groups compared to the SHAM group on day 6 (t=2.91, 3.52, 3.97, 2.71, P<0.05), with no significant differences among the three groups by day 10 (P>0.05). Egg-laying capacity significantly reduced in both irradiated groups compared to the SHAM group (t=1.72, 5.54, P<0.05), while the UHDR group exhibited higher fecundity than the CONV group (t=-5.99, P<0.05). Lifespan was significantly shortened in the CONV group compared to the SHAM group (χ2=8.49, P<0.05), whereas the survival time of the UHDR group was not significantly differ from that of the SHAM group (P>0.05). Conclusions Exposure to a conventional electron beam result in developmental delays, reduced mobility, decreased fecundity, and a shortened lifespan in C. elegans. However, only slight side effects were observed when C. elegans were exposed to an ultra-high dose rate pulsed electron beam at the same dosage. |
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