| Li Mingfang,Zhang Lingyu,Cai Lina,et al.Impacts of X-rays at varying doses on liver injury and oxidative stress in mice[J].Chinese Journal of Radiological Medicine and Protection,2024,44(9):734-740 |
| Impacts of X-rays at varying doses on liver injury and oxidative stress in mice |
| Received:February 05, 2024 |
| DOI：10.3760/cma.j.cn112271-20240205-00048 |
| KeyWords:X-rays Radiation-induced liver injury Oxidative stress |
| FundProject:国家自然科学基金(81302387);广东省基础与应用基础研究基金(2019A1515011969、2022A1515012421、2023A1515010414);广东省医学科研基金项目(B2020119,C2022034);广州市科技计划项目(202002030031) |
| Author Name | Affiliation | E-mail | | Li Mingfang | Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Zhang Lingyu | Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China
Guangzhou Medical University, School of Public Health, Guangzhou 511436, China | | | Cai Lina | Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Zhang Sufen | Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Cai Yashi | Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Yang Yuhua | Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Chen Huifeng | Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Zou Jianming | Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | | | Huang Weixu | Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China | 18927588197@163.com |
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| Abstract:: |
| Objective To investigate the changes in liver injury and oxidative-antioxidant level in mice exposed to X-rays at varying doses. Methods Fifty-four 8-week-old male C57BL/6J mice were divided into three groups, namely the control, 2 Gy irradiation, and 4 Gy irradiation groups. Then, each of the groups was further divided by days post-irradiation (i.e., 1, 3, and 7 d), and so nine sub-groups (n = 6). After irradiation was performed as planned, all the mice were dissected and weighed, and their liver indexes were calculated to determine any histopathological changes in the liver. The peripheral blood cell count and the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were detected. Furthermore, spectrophotometry was also used to determine the superoxide dismutase (SOD) activity, the malondialdehyde (MDA) concentration, and the reduced glutathione (GSH) concentration in liver tissues. Results Compared to the control group, mice undergoing irradiation exhibited a significant reduction in body weight (F = 84.03, 27.11, 25.50, P< 0.001), but significantly increased liver indexes (F = 28.40, 17.75, P<0.001) at 1, 3, and 7 d post-irradiation. Pathological observations of these mice revealed liver injury, which proved related to dose and time course. The counts of leukocytes, neutrophils, and lymphocytes in peripheral blood decreased significantly (F = 8.42-22.91, P< 0.05), trending downward with an increase in the radiation dose. For mice in the 4 Gy irradiation group, their AST and ALT levels increased significantly at 1 d post-irradiation (H = 7.24, 7.82, P< 0.05), and their ALP levels rose notably at 1 and 3 d post-irradiation (F = 11.86, 9.75, P< 0.05). Furthermore, their MDA and SOD levels initially rose and then dropped but their GSH levels exhibited an opposite trend at 1, 3, and 7 d post-irradiation. There was a positive correlation between their MDA levels in the liver and the degree of damage to histopathological lesions at 1, 3, and 7 d post-irradiation (r= 0.30, P < 0.001). Conclusions A model for radiation-induced liver injury of mice was preliminarily established in this study. It can be concluded that X-rays at varying doses affect the severity of liver injury, pathological grade, peripheral blood cell count, liver function index, and liver oxidative and antioxidant levels of mice, presenting a certain relationship between dose and time course effects. |
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