Chen Jie,Liu Mengmeng,Gao Ling.Establishing a three-dimensional model for estimating partial-body irradiation doses based on γ-H2AX foci detection[J].Chinese Journal of Radiological Medicine and Protection,2024,44(3):188-193
Establishing a three-dimensional model for estimating partial-body irradiation doses based on γ-H2AX foci detection
Received:September 11, 2023  
DOI:10.3760/cma.j.cn112271-20230911-00083
KeyWords:γ-H2AX  Partial-body irradiation  Dose estimation  Three-dimensional model
FundProject:北京市自然科学基金(7162139,7202137);国家自然科学基金(82273551,31570852);中国疾病预防控制中心辐射防护与核安全医学所青年科学研究所长基金(2020-04)
Author NameAffiliationE-mail
Chen Jie Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China  
Liu Mengmeng Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China  
Gao Ling Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China gaoling@nirp.chinacdc.cn 
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Abstract::
      Objective To construct a three-dimensional time-dose-response model for dose estimation and validate its feasibility. Methods Based on a random number table, mice were divided into 0, 2, 4, 6, and 8 Gy groups for whole-body X-ray irradiation, with each group consisting of three mice. Hair follicle cells of whiskers were sampled at 1, 6, and 24 h after the irradiation. After immunofluorescence staining, the numbers of γ-H2AX foci at different time points from 1 to 24 h post-irradiation were observed using a confocal laser scanning microscope. The average numbers of γ-H2AX foci observed were corrected using the Dolphin’s model, followed by the fitting of dose-response curves. Using the R software, the equations and surfaces of the three-dimensional model for partial-body irradiation were established using the irradiation doses, post-irradiation time, and the corrected average numbers of γ-H2AX foci. Results The average number of γ-H2AX foci increased with dose at fixed time points 1, 6, and 24 h but decreased with irradiation time at fixed doses 2, 4, 6, and 8 Gy. The dose-response curve equations of partial-body irradiation were fitted as follows: YF = 2.853+3.775 D, R2= 0.928, at 1 h after the irradiation; YF = 0.144+2.775 D, R2= 0.903, at 6 h after the irradiation; YF = 0.066+2.472 D, R2= 0.85, at 24 h after the irradiation. The three-dimensional model equation fitted was YF = 6.837 t-1.728+3.113 t-0.071 D, R2=0.897. Substituting different post-irradiation time points into the three-dimensional surface model appeared as a two-dimensional linear model. By substituting the number of γ-H2AX foci and irradiation time into the linear and the three-dimensional models, both models yielded relative deviations between the estimated and actual radiation doses of 30% or less. Conclusions The three-dimensional time-dose-response model, established by using the number of γ-H2AX foci to estimate partial-body irradiation doses, can be preliminarily applied for dose estimation at all time points 1-24 h after irradiation.
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