An Lu,Chen Xiaoming,Li Can,Yao Jian,Zhang Shuyu,Cao Jianping,Gong Zhenhua,Yu Daojiang.Conversion of stromal vascular fraction in the microenvironment of radiation-induced skin injuries and its clinical implications[J].Chinese Journal of Radiological Medicine and Protection,2024,44(3):181-187
Conversion of stromal vascular fraction in the microenvironment of radiation-induced skin injuries and its clinical implications
Received:March 27, 2023  
DOI:10.3760/cma.j.cn112271-20230327-00100
KeyWords:Stromal vascular fraction  Radiation-induced skin injury  Conversion
FundProject:国家自然科学基金(82373523,32071238,U1967220);中核集团(青年英才)科研项目—菁英项目(CNNC2021136);中核医疗第二届"核医科技创新"项目(ZHYLZD2022004);四川省科技计划项目(2021YJ0258);成都市医学科研课题(2021085);南京医科大学康达学院科研发展基金(KD2022KYJJZD021);南通市卫健委医学科研课题(MS2022017);南通市医学重点人才项目(2021);江苏省333人才项目(2022)
Author NameAffiliationE-mail
An Lu Department of Burn and Plastic Surgery, Huai'an 82 Hospital, Huai'an 223001, China  
Chen Xiaoming Department of Plastic and Burn Surgery, the Second Affiliated Hospital of Chengdu Medical College, Chengdu 610051, China  
Li Can Department of Plastic and Burn Surgery, the Second Affiliated Hospital of Chengdu Medical College, Chengdu 610051, China  
Yao Jian Department of Burn and Plastic Surgery, Nantong First People's Hospital, Nantong 226001, China  
Zhang Shuyu Department of Plastic and Burn Surgery, the Second Affiliated Hospital of Chengdu Medical College, Chengdu 610051, China
West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China 
 
Cao Jianping State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University, Suzhou 215123, China  
Gong Zhenhua Department of Burn and Plastic Surgery, Nantong First People's Hospital, Nantong 226001, China  
Yu Daojiang Department of Plastic and Burn Surgery, the Second Affiliated Hospital of Chengdu Medical College, Chengdu 610051, China
West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China 
ydj51087@163.com 
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Abstract::
      Objective To investigate the conversion of stromal vascular fraction (SVF) in the microenvironment of radiation-induced skin injuries to provide guidance for clinical applications. Methods Based on a random number table, C57BL/6N mice were categorized into four groups: the blank control, negative control, acute injury, and chronic injury groups, with each group containing 25 mice. The backs of mice in the blank control, acute injury, and chronic injury groups were exposed to 15 Gy X-ray irradiation. Then, the mice in the negative control, acute injury, and chronic injury groups were injected subcutaneously with the SVF derived from B6/G-R mice. The survival of these mice was observed 1, 3, 7, 14, and 21 d after the injection through fluorescence tracing and in vivo imaging. Accordingly, the clinical SVF injection regimens were optimized based on the experimental result of mice. Finally, local SVF injection was performed on different frequencies for patients in different wound conditions, with the efficacy being observed. Results The fluorescence of SVF was observed from the tissue slices of the acute injury, chronic injury, and negative control groups 14 d post-injection. The result showed that the fluorescence intensity of SVF 1, 3, and 7 d post-injection was in the order of the negative control group > the acute injury group > the chronic injury group. The acute injury group ranked at the top and the chronic injury group remained at the bottom 14 d after the injection. The fluorescence of SVF in each group was barely detected 21 d after the injection. Compared to the negative control group, the acute injury group exhibited statistical differences only 14 d post-injection (t = 4.11, P < 0.05), while the chronic injury group displayed statistical differences 1, 3, 7, and 14 d after the injection (t = 3.88-5.74, P < 0.05). Furthermore, the acute injury group exhibited significantly higher fluorescence intensity of SVF than the chronic injury group (t = 4.73-8.38, P < 0.05). The half-life of SVF for the negative control, acute injury, and chronic injury groups was 6.336, 6.014, and 2.163 d, respectively. As indicated by the application of SVF transplantation based on traditional surgical protocols in the clinical trial, SVF can significantly promote wound repair, with earlier SVF transplantation being more beneficial for wound healing. Conclusions The conversion of SVF differs in the microenvironments of acute and chronic radiation-induced skin injuries. This can serve as an essential guide for the administration timing and injection frequency of SVF in clinical applications.
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