| Luo Hui,Ma Leijie,Mao Ronghu,Li Na,Xue Fumin,Wang Xuenan,Lei Hongchang,Song Shuai,Ge Hong,Yang Chengliang.Effects and mechanism of ultra-high dose rate irradiation in reducing radiation damage to zebrafish embryos[J].Chinese Journal of Radiological Medicine and Protection,2024,44(3):174-180 |
| Effects and mechanism of ultra-high dose rate irradiation in reducing radiation damage to zebrafish embryos |
| Received:October 23, 2023 |
| DOI：10.3760/cma.j.cn112271-20231023-00135 |
| KeyWords:Ultra-high dose rate irradiation Zebrafish Embryo Oxidative stress Radiation damage |
| FundProject:河南省科技攻关计划项目(212102310619);河南省医学科技攻关计划项目(LHGJ20210171) |
| Author Name | Affiliation | E-mail | | Luo Hui | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Ma Leijie | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Mao Ronghu | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Li Na | School of Radiology, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an 271021, China | | | Xue Fumin | Children's Hospital Affiliated of Zhengzhou University, Zhengzhou 450018, China | | | Wang Xuenan | School of Radiology, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an 271021, China | | | Lei Hongchang | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Song Shuai | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Ge Hong |
1 Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China
2 School of Radiology, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an 271021, China
3 Children's Hospital Affiliated of Zhengzhou University, Zhengzhou 450018, China
| zlyygehong0199@zzu.edu.cn | | Yang Chengliang |
1 Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China
2 School of Radiology, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an 271021, China
3 Children's Hospital Affiliated of Zhengzhou University, Zhengzhou 450018, China
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| Abstract:: |
| Objective To conduct a comparative analysis of the radiation damage to zebrafish embryos and the associated biological mechanism after ultra-high dose rate (FLASH) and conventional dose rate irradiation. Methods Zebrafish embryos at 4 h post-fertilization were exposed to conventional and FLASH irradiation (9 MeV electron beam). The mortality and hatchability of zebrafish after radiation exposure were recorded. Larvae at 96 h post-irradiation underwent morphological scoring, testing of reactive oxygen species (ROS) levels, and analysis of changes in oxidative stress indicators. Results Electron beam irradiation at doses of 2-12 Gy exerted subtle effects on the mortality and hatchability of zebrafish embryos. However, single high-dose irradiation (≥ 6 Gy) could lead to developmental malformation of larvae, with conventional irradiation showing the most significant effects (t = 0.87-9.75, P < 0.05). In contrast, after FLASH irradiation (≥ 6 Gy), the ROS levels in zebrafish and its oxidative stress indicators including superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were significantly reduced (t = 0.42-15.19, P < 0.05). There was no statistically significant difference in ROS levels in incubating solutions after conventional and FLASH irradiation (P > 0.05). Conclusions Compared to conventional irradiation, FLASH irradiation can reduce radiation damage to zebrafish embryos, and this is in a dose-dependent manner. The two irradiation modes lead to different oxidative stress levels in zebrafish, which might be a significant factor in the reduction of radiation damage with FLASH irradiation. |
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