Luo Hui,Ma Leijie,Mao Ronghu,Li Na,Xue Fumin,Wang Xuenan,Lei Hongchang,Song Shuai,Ge Hong,Yang Chengliang.Effects and mechanism of ultra-high dose rate irradiation in reducing radiation damage to zebrafish embryos[J].Chinese Journal of Radiological Medicine and Protection,2024,44(3):174-180
Effects and mechanism of ultra-high dose rate irradiation in reducing radiation damage to zebrafish embryos
Received:October 23, 2023  
DOI:10.3760/cma.j.cn112271-20231023-00135
KeyWords:Ultra-high dose rate irradiation  Zebrafish  Embryo  Oxidative stress  Radiation damage
FundProject:河南省科技攻关计划项目(212102310619);河南省医学科技攻关计划项目(LHGJ20210171)
Author NameAffiliationE-mail
Luo Hui Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China  
Ma Leijie Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China  
Mao Ronghu Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China  
Li Na School of Radiology, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an 271021, China  
Xue Fumin Children's Hospital Affiliated of Zhengzhou University, Zhengzhou 450018, China  
Wang Xuenan School of Radiology, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an 271021, China  
Lei Hongchang Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China  
Song Shuai Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China  
Ge Hong 
1 Department of Radiation Oncology
, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China

2 School of Radiology
, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an 271021, China

3 Children's Hospital Affiliated of Zhengzhou University
, Zhengzhou 450018, China
 
zlyygehong0199@zzu.edu.cn 
Yang Chengliang 
1 Department of Radiation Oncology
, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China

2 School of Radiology
, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an 271021, China

3 Children's Hospital Affiliated of Zhengzhou University
, Zhengzhou 450018, China
 
 
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Abstract::
      Objective To conduct a comparative analysis of the radiation damage to zebrafish embryos and the associated biological mechanism after ultra-high dose rate (FLASH) and conventional dose rate irradiation. Methods Zebrafish embryos at 4 h post-fertilization were exposed to conventional and FLASH irradiation (9 MeV electron beam). The mortality and hatchability of zebrafish after radiation exposure were recorded. Larvae at 96 h post-irradiation underwent morphological scoring, testing of reactive oxygen species (ROS) levels, and analysis of changes in oxidative stress indicators. Results Electron beam irradiation at doses of 2-12 Gy exerted subtle effects on the mortality and hatchability of zebrafish embryos. However, single high-dose irradiation (≥ 6 Gy) could lead to developmental malformation of larvae, with conventional irradiation showing the most significant effects (t = 0.87-9.75, P < 0.05). In contrast, after FLASH irradiation (≥ 6 Gy), the ROS levels in zebrafish and its oxidative stress indicators including superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were significantly reduced (t = 0.42-15.19, P < 0.05). There was no statistically significant difference in ROS levels in incubating solutions after conventional and FLASH irradiation (P > 0.05). Conclusions Compared to conventional irradiation, FLASH irradiation can reduce radiation damage to zebrafish embryos, and this is in a dose-dependent manner. The two irradiation modes lead to different oxidative stress levels in zebrafish, which might be a significant factor in the reduction of radiation damage with FLASH irradiation.
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