| Li Kejun,Du Liqing,Liu Qiang,Song Huijuan.Effects of glycosylated nanoparticles on radiation-induced the polarization of macrophages in early injured lung tissue[J].Chinese Journal of Radiological Medicine and Protection,2023,43(11):858-865 |
| Effects of glycosylated nanoparticles on radiation-induced the polarization of macrophages in early injured lung tissue |
| Received:February 20, 2023 |
| DOI：10.3760/cma.j.cn112271-20230220-00172 |
| KeyWords:Radiation-induced lung injury Pulmonary macrophages M2 polarization Reactive oxygen species Inflammation |
| FundProject:国家自然科学基金(82001954,32071241,81972976,32171239);辐射防护实验室开放基金(CIRP-DTRI20220202);中国医学科学院医疗健康科技创新项目(2022-I2M-2-003) |
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| Abstract:: |
| Objective To explore the effects of glycosylated nanoparticles on early radiation responses including the generation of reactive oxygen species (ROS), the polarization of pulmonary macrophages, and secretion of inflammatory cytokines in mice lung tissues.Methods Twenty mice were randomly divided into control group, drug administration group, irradiation group and irradiation + drug administration group. The irradiation group and irradiation + drug administration group were subjected to whole lung irradiation with X-rays. The antioxidant ability of glycosylated nanoparticles was characterized using ROS indicator (CM-H2DCFDA). The M2 polarization of pulmonary macrophages was detected by flow cytometry and PCR. The mRNA and protein expression levels of inflammatory cytokines were investigated by PCR and ELISA assay, respectively.Results Compared with the irradiation group, the intensity of ROS fluorescence signals was significantly lower (t=15.76, P < 0.05), the proportion of M2-type macrophages was significantly higher (t=2.89, P < 0.05), the expression level of arginase 1 (ARG-1) gene was elevated, and the expression levels of tumour necrosis factor α (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6) inflammatory factors were significantly reduced (t=3.32, 2.90, 2.85, 4.55, 2.88, P < 0.05) in the lung tissues of irradiation + treatment group.Conclusions Glycosylated nanoparticles can effectively scavenge ROS, trigger polarization of M2 macrophage, dampen inflammatory responses, and thus potentially alleviate radiation-induced lung injury. |
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