| Zhang Jun,Hu Wentao,Xu Zhiming,Cheng Xinyang,Zhang Yongsheng,Cao Zhifei.Comparative analysis of the temporal characteristics of plasma metabolites in glioma mice after ultra-high dose rate radiation and conventional radiation[J].Chinese Journal of Radiological Medicine and Protection,2023,43(10):759-765 |
| Comparative analysis of the temporal characteristics of plasma metabolites in glioma mice after ultra-high dose rate radiation and conventional radiation |
| Received:March 30, 2023 |
| DOI：10.3760/cma.j.cn112271-20230330-00102 |
| KeyWords:Ultra-high dose rate radiation Conventional radiation Glioma Biomarker |
| FundProject:省部共建放射医学与辐射防护国家重点实验室开放课题(GZK1202221);苏州大学附属第二医院核技术医学应用重点人才项目(XKTJ-HRC2021002) |
| Author Name | Affiliation | E-mail | | Zhang Jun | Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Hu Wentao | State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University, Suzhou 215123, China | | | Xu Zhiming | Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Cheng Xinyang | Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Zhang Yongsheng | Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Cao Zhifei | Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | hunancao@163.com |
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| Abstract:: |
| Objective To investigate the effects of ultra-high dose rate radiation (FLASH-RT) and conventional radiation (CONV-RT) on plasma metabolites in glioma mice. Methods Tocally 21 male C57BL/6J mice bearing intracranial glioma xenograft were randomly divided into healthy control group (n=3), CONV-RT group (n=9) and FLASH-RT group (n=9). The CONV-RT group was administered a single dose of 24 Gy radiation on the head of mice at a dose rate of 0.4 Gy/s, and the FLASH-RT group was administered a single dose of 24 Gy radiation on the head of mice at a dose rate of 60 Gy/s, and the healthy control group was given 0 Gy pseudoradiation under the same condition. Mice blood was collected through the inner canthus vein for plasma separation at 1, 3 and 7 d after radiation in the two radiation groups, and the blood plasma of healthy control group was collected at 7 days after sham radiation. The changes in plasma metabolites were detected by the non-targeted metabolomics based on liquid chromatography mass spectrometry tandem platform. Results After irradiation, the metabolites in plasma of two irradiation groups had significant difference. Compared with the healthy control group, 12 and 5 differential metabolites were screened out in the FLASH-RT group and CONV-RT group at three time points, respectively. The difference of plasma metabolites had the largest value at 1 day and decreased at 3 and 7 d after radiation. The arachidonic acid and isovaleric acid, involving arachidonic acid metabolism, biosynthesis of unsaturated fatty acids, and tyrosine metabolism pathways were screened in both FLASH-RT group and CONV-RT group, and the 10 differential metabolites, mainly involving energy metabolism and redox reactions, only existed in the FLASH-RT group. Conclusions Arachidonic acid and isovaleric acid may be the common sensitive biomarkers to different radiation patterns, which provides ideas for further exploring the molecular mechanism of FLASH-RT in the treatment of glioma. |
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