| Zhang Songling,Zhu Changchun,Feng Guoxing,Fan Saijun.Elevated miR-27b-3p enhances the radioresistance by targeting PLK2 in breast cancer[J].Chinese Journal of Radiological Medicine and Protection,2023,43(6):409-417 |
| Elevated miR-27b-3p enhances the radioresistance by targeting PLK2 in breast cancer |
| Received:October 14, 2022 |
| DOI：10.3760/cma.j.cn112271-20221014-00408 |
| KeyWords:miR-27b-3p PLK2 Breast Cancer MCF-7 Radioresistance |
| FundProject:中国医学科学院医学与健康科技创新工程重大协同创新项目（2021-I2M-1-042） |
| Author Name | Affiliation | E-mail | | Zhang Songling | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | Zhu Changchun | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | Feng Guoxing | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | Fan Saijun | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | fansaijun@irm-cams.ac.cn |
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| Abstract:: |
| Objective To investigate the effect of miR-27b-3p on radiation resistance of breast cancer cells.Methods The relative expression levels of miR-27b-3p in normal tissues and breast cancer tissues were analyzed through GEO database and verified by the qRT-PCR assay. Cloning formation, immunofluorescence, and EDU assay were used to assess the functions of miR-27b-3p on radioresistance of breast cancer cells. The luciferase reporter assay was used to verify whether miR-27b-3p directly targeted PLK2 mRNA. A rescue experiment was performed to identify the influence of PLK2 overexpression on miR-27b-3p regulated radioresistance.Results The expression level of miR-27b-3p in both breast cancer tissues (t=2.99, P<0.01) and breast cancer cells (t=21.21, 32.88, P<0.05) was significantly higher than those in normal breast tissues and cells, especially, it was elevated in radioresistant MCF-7R cells (t=25.63, P<0.05). Overexpression of miR-27b-3p enhanced the cloning efficiency of MCF-7 cells (t=10.32, P<0.05), and had a protective effect on the proliferation of irradiated MCF-7 cells (t=8.77, 8.26, 8.03, P<0.05). But interference of miR-27b-3p reduced the cloning efficiency and proliferation of MCF-7R cells (t=40.00, P<0.05) after irradiation with different doses(t=8.54, 8.32, 8.23, P<0.05). Moreover, PLK2 was verified to be a direct target of miR-27b-3p, and overexpression of PLK2 inhibited miR-27b-3p-mediated radioresistance of breast cancer cells (MCF-7:t=9.66, P<0.05; MCF-7R:t=6.42, P<0.05).Conclusions miR-27b-3p contributes to the radioresistance of breast cancer cells by targeting PLK2. |
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