| Kong Xiaoxu,Feng Zhihua,Wang Xuejia,He Ganghua,Pan Ting,Xu Zhengtao,Ye Yumeng,Hao Yanhui,Zuo Hongyan,Li Yang.Mechanism of the therapeutic effect of Tetrandrine on striatum injury caused by microwave radiation[J].Chinese Journal of Radiological Medicine and Protection,2023,43(5):328-334 |
| Mechanism of the therapeutic effect of Tetrandrine on striatum injury caused by microwave radiation |
| Received:January 13, 2023 |
| DOI：10.3760/cma.j.cn112271-20230113-00010 |
| KeyWords:Tetrandrine Microwave radiation Striatal Voltage-gated calcium channel |
| FundProject:北京市自然科学基金面上项目(722225) |
| Author Name | Affiliation | E-mail | | Kong Xiaoxu | Academy of Life Sciences, Anhui Medical University, Hefei 230032, China | | | Feng Zhihua | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Wang Xuejia | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | He Ganghua | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Pan Ting | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Xu Zhengtao | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Ye Yumeng | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Hao Yanhui | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Zuo Hongyan | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Li Yang | Academy of Life Sciences, Anhui Medical University, Hefei 230032, China | leeyoung33@163.com |
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| Abstract:: |
| Objective To study the therapeutic effect Tetrandrine (TET) on striatal injury caused by microwave radiation and underlying mechanism. Methods C57BL/6N mice were randomly divided into blank control group (C), radiation control group (R), TET group (TET) and TET combined with radiation group (TET+R). The mice of radiation group were exposed to 2.856 GHz 8 mW/cm2 microwave on whole-body for 15 min. TET (60 mg/kg) was injected intraperitoneally once a day for 3 consecutive days. The TET structure was verified by ultraviolet spectrophotometry. The open field experiment was used to detect the change of anxiety in mice. Histopathological and ultrastructural changes of the striatum were observed by light microscopy and transmission electron microscopy (TMT). Quantitative real-time PCR (qPCR) was used to detect gene expression changes of voltage-gated calcium channel (VGCC) subtype in the striatum. Results The open field experiments showed that the time and distance of mice to explore the central region after microwave radiation were significantly lower than that before radiation (t=4.60, 5.18, P<0.01), and the TET administration significantly improved these changes (F=1.43, 4.37, P < 0.05). 7 d after microwave radiation, some neuronal nuclei in the striatum of mice contracted and could be stained deeply, which was more obvious in the globus pallidus area. The partial neuronal apoptosis, swelling and cavitation of glial cell mitochondria, blurring of synaptic gaps, and widening of perivascular gaps in the striatum were observed by TMT. The above lesions were significantly rescued after TET administration. But both microwave radiation and TET administration had no significant effect on the gene expressions of striatal VGCC (P > 0.05). Conclusions TET has a therapeutic effect on anxiety-like behavior and structural damage of striatum caused by microwave radiation, which is independent of the expression of striatal VGCC genes. |
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