| Tian Xuelei,Liu Haixiang,Lu Xue,Tian Mei,Liu Qingjie.Effect of circular RNA hsa_circZDHHC21_004 on the proliferation of the human small intestinal epithelial cells HIEC-6 exposed to 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2023,43(5):321-327 |
| Effect of circular RNA hsa_circZDHHC21_004 on the proliferation of the human small intestinal epithelial cells HIEC-6 exposed to 60Co γ-rays |
| Received:January 04, 2023 |
| DOI：10.3760/cma.j.cn112271-20230104-00002 |
| KeyWords:Circular RNA Human small intestinal epithelial cell Cell proliferation Ionizing radiation |
| FundProject:国家自然科学基金(82173463) |
| Author Name | Affiliation | E-mail | | Tian Xuelei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Haixiang | Medical examination center, Beijing Nuclear Industry Hospital, Beijing 100045, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Mei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | liuqingjie@nirp.chinacdc.cn |
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| Abstract:: |
| Objective To study the influence of circular RNA hsa_circZDHHC21_004 on the proliferation of human small intestinal epithelial cells HIEC-6 after 60Co γ-rays exposure. Methods HIEC-6 cells were exposed to 60Co γ-rays at 0, 5, 10, and 15 Gy with a dose rate of 1 Gy/min. The expression level of hsa_circZDHHC21_004 in the irradiated HIEC-6 cell was detected. Hsa_circZDHHC21_004 was knocked-down to investigate the influences of hsa_circZDHHC21_004 on the proliferation of irradiated HIEC-6 cells by CCK-8 assay and colony formation assay. Results The expression level of hsa_circZDHHC21_004 in HIEC-6 cells was upregulated by (1.00±0.24), (1.34±0.28), (1.85±0.31), and (2.80±0.64) times of control after 0, 5, 10, and 15 Gy irradiation, respectively and there were significant difference between 10 or 15 Gy group and 0 Gy group (F=10.86, P=0.008). Knockdown of hsa_circZDHHC21_004 significantly increased the proliferation rate of HIEC-6 cells at 24, 48, and 72 h after 10 Gy irradiation compared with non-irradiated control (t=-6.25, -5.83, -7.75, P < 0.001). Under 2 and 5 Gy irradiation, the clone formation rates of the hsa_circZDHHC21_004 knockdown cells were significantly higher than those of the control (t=-7.45,-8.83, P<0.01). Conclusions Hsa_circZDHHC21_004 is increased after irradiation and influenced the proliferation of irradiated HIEC-6 cells. |
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