| He Xiulan,Luo Zhibin.Protective effect and mechanism of ophiopogonin D on radiation-induced lung injury in mice[J].Chinese Journal of Radiological Medicine and Protection,2023,43(4):248-255 |
| Protective effect and mechanism of ophiopogonin D on radiation-induced lung injury in mice |
| Received:September 12, 2022 |
| DOI：10.3760/cma.j.cn112271-20220912-00370 |
| KeyWords:Ophiopogonin D Radiation induced lung injury Oxidative stress Apoptosis p53 |
| FundProject: |
| Author Name | Affiliation | E-mail | | He Xiulan | Department of Oncology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
Department of Oncology, Chongqing General Hospital, Chongqing 400010, China | | | Luo Zhibin | Department of Oncology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
Department of Oncology, Chongqing General Hospital, Chongqing 400010, China | luozhibincq@126.com |
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| Abstract:: |
| Objective To investigate the protective effect and mechanism of ophiopogonin D on lung injury induced by radiation in mice.Methods A total of 60 female C57BL/6 mice were randomly divided into 4 groups: control group, irradiation group, irradiation+ophiopogonin D group and irradiation+dexamethasone group, with 15 mice in each group. The mice were irradiated with a single dose of 6 MV X-rays of 15 Gy. Three days before irradiation, the mice in irradiation+ophiopogonin D group were intraperitoneally injected with 10 mg/kg ophiopogonin D solution. The mice in irradiation+dexamethasone group were intraperitoneally injected with 10 mg/kg dexamethasone solution. The mice in control group and irradiation group were intraperitoneally injected with normal saline once a day until 1 week after irradiation. Tissue samples were collected at 3 d, 1 week, and 6 weeks post-irradiation. Hematoxylin-eosin (HE) staining and Masson's trichrome staining were used to observe the pathological changes of lung tissue. The expressions of 8-hydroxy-deoxyguanosine (8-OHdG), p53, p53 up-regulated apoptosis factor (PUMA), cysteine aspartate proteolytic enzyme-3 (caspase-3), Collagen Ⅰ and Collagen Ⅲ were observed by immunohistochemistry. Western blot was used to verify the expressions of apoptosis related proteins including p53, PUMA and caspase-3.Results HE staining of lung tissue showed that ophiopogonin D could reduce hemorrhage, exudation, edema and inflammatory infiltration in lung tissue 1 week post irradiation. Moreover, ophiopogonin D reduced the expression of 8-OHdG (t=8.39, P < 0.05), the oxidative stress, and the expressions of p53, PUMA, caspase-3 apoptosis-related proteins (t=12.60, 5.92, 7.00, P < 0.05), and inhibited the apoptosis of alveolar epithelial cells and alleviated other damage in the irradiated lung tissue 1 week post-irradiation. Ophiopogonin D also reduced collagen deposition in lung tissue 6 weeks after irradiation, and reduced the expression of transforming growth factor (TGF-β1) (t=9.32, 8.97, 6.83, P < 0.05) and interleukin-6 (t=8.22, 7.80, 8.28, P < 0.05) in the blood of mice at 3 d, 1 week, and 6 weeks after irradiation. At 6 weeks after exposure,ophiopogonin D reduced the production of Collagen Ⅰ and Collagen Ⅲ in the lung interstitium (t=6.41, 7.50, P < 0.05), and alleviated the pulmonary fibrosis in the late stage of radiation.Conclusions Ophiopogonin D has protective effects on lung injury caused by radiation, including the alleviation of early radiation pneumonia and late pulmonary fibrosis, by reducing oxidative stress, the expression of inflammation-related factors, apoptosis of lung tissue, and collagen production. |
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