| Luo Hui,Yuan Qigang,Phyllis Zhang,Ma Leijie,Mao Ronghu,Lei Hongchang,Sun Yanan,Song Shuai,Wang Xiaohui,Ge Hong.Ultra-high dose rate irradiation induced DNA strand break in plasmid DNA[J].Chinese Journal of Radiological Medicine and Protection,2023,43(3):161-167 |
| Ultra-high dose rate irradiation induced DNA strand break in plasmid DNA |
| Received:November 22, 2022 |
| DOI：10.3760/cma.j.cn112271-20221122-00453 |
| KeyWords:Ultra-high dose rate irradiation DNA strand breaks Physioxia Free radical Relative biological effectiveness |
| FundProject:国家自然科学基金（81773230）；河南省科技攻关计划项目（212102310619） |
| Author Name | Affiliation | E-mail | | Luo Hui | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Yuan Qigang | School of Mathematics and Statistics, North China University of Water Resources and Electric Power, Zhengzhou 450046, China | | | Phyllis Zhang | University of Hong Kong, Hong Kong 999077, China | | | Ma Leijie | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Mao Ronghu | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Lei Hongchang | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Sun Yanan | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Song Shuai | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Wang Xiaohui | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Ge Hong | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | zlyygehong0199@zzu.edu.cn |
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| Abstract:: |
| Objective To compare the effects on DNA strand break induced by ultra-high dose rate (FLASH) electron beam and conventional irradiation, and investigate whether FLASH effect was correlated with a reduction of radiation response.Methods Aqueous pBR322 plasmid was treated with FLASH (125 Gy/s) and conventional irradiation (0.05 Gy/s) under physioxia (4% O2) and normoxia (21% O2). Open circle DNA and linear DNA were detected by agarose gel electrophoresis, and the plasmid DNA damage was quantified with an established mathematical model to calculate the relative biological effect (RBE) of DNA damage. In some experiments, Samwirin A (SW) was applied to scavenge free radicals generated by ionizing radiation.Results Under physioxia, the yields of DNA strand breakage induced by both FLASH and conventional irradiation had a dose-dependent manner. FLASH irradiation could significantly decrease radiation-induced linear DNA compared with conventional irradiation (t=5.28, 5.79, 7.01, 7.66, P < 0.05). However, when the aqueous plasmid was pretreated with SW, there was no difference of DNA strand breakage between FLASH and conventional irradiation (P > 0.05). Both of the yields of open circle DNA and linear DNA had no difference caused by FLASH and conventional radiotherapy at normoxia, but were significantly higher than those under physioxia. In addition, the yields of linear DNA and open circle DNA induced by FLASH irradiation per Gy were (2.78±0.03) and (1.85±0.17) times higher than those of conventional irradiation, respectively.Conclusions FLASH irradiation attenuated radiation-induced DNA damage since a low production yield of free radical in comparison with conventional irradiation, and hence the FLASH effect was correlated with oxygen content. |
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