| Guan Hongdan,Zheng Rong,Guan Bingjie,Lin Yuping,Wang Bisi,Xu Benhua,Song Jianyuan.Effect of down-regulation of FABP5 on radiation damage of human keratinocytes[J].Chinese Journal of Radiological Medicine and Protection,2023,43(1):8-14 |
| Effect of down-regulation of FABP5 on radiation damage of human keratinocytes |
| Received:September 21, 2022 |
| DOI：10.3760/cma.j.cn112271-20220921-00381 |
| KeyWords:Skin Fatty acid binding protein 5 Radiosensitivity Radiation protection PI3K/AKT signaling pathway |
| FundProject:福建省卫生健康中青年骨干人才培养项目(2019-ZQN-44) |
| Author Name | Affiliation | E-mail | | Guan Hongdan | Department of Radiation Oncology, Fujian Medical University Union Hospital, Fujian Key Laboratory of Intelligent Imaging and Precision Radiotherapy for Tumors(Fujian Medical University), Clinical Research Center for Radiology and Radiotherapy of Fujian Province(Digestive, Hematologicaland Breast Malignancies), Fuzhou 350001, China | | | Zheng Rong | Department of Radiation Oncology, Fujian Medical University Union Hospital, Fujian Key Laboratory of Intelligent Imaging and Precision Radiotherapy for Tumors(Fujian Medical University), Clinical Research Center for Radiology and Radiotherapy of Fujian Province(Digestive, Hematologicaland Breast Malignancies), Fuzhou 350001, China | | | Guan Bingjie | Department of Radiation Oncology, Fujian Medical University Union Hospital, Fujian Key Laboratory of Intelligent Imaging and Precision Radiotherapy for Tumors(Fujian Medical University), Clinical Research Center for Radiology and Radiotherapy of Fujian Province(Digestive, Hematologicaland Breast Malignancies), Fuzhou 350001, China | | | Lin Yuping | Department of Radiation Oncology, Fujian Medical University Union Hospital, Fujian Key Laboratory of Intelligent Imaging and Precision Radiotherapy for Tumors(Fujian Medical University), Clinical Research Center for Radiology and Radiotherapy of Fujian Province(Digestive, Hematologicaland Breast Malignancies), Fuzhou 350001, China | | | Wang Bisi | Department of Radiation Oncology, Fujian Medical University Union Hospital, Fujian Key Laboratory of Intelligent Imaging and Precision Radiotherapy for Tumors(Fujian Medical University), Clinical Research Center for Radiology and Radiotherapy of Fujian Province(Digestive, Hematologicaland Breast Malignancies), Fuzhou 350001, China | | | Xu Benhua | Department of Radiation Oncology, Fujian Medical University Union Hospital, Fujian Key Laboratory of Intelligent Imaging and Precision Radiotherapy for Tumors(Fujian Medical University), Clinical Research Center for Radiology and Radiotherapy of Fujian Province(Digestive, Hematologicaland Breast Malignancies), Fuzhou 350001, China | | | Song Jianyuan | Department of Radiation Oncology, Fujian Medical University Union Hospital, Fujian Key Laboratory of Intelligent Imaging and Precision Radiotherapy for Tumors(Fujian Medical University), Clinical Research Center for Radiology and Radiotherapy of Fujian Province(Digestive, Hematologicaland Breast Malignancies), Fuzhou 350001, China | songjianyuan@163.com |
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| Abstract:: |
| Objective To investigate the effects of down-regulation of FABP5 (fatty acid binding protein 5) on radiation damage of skin cells, and explore underlying mechanism.Methods A lentiviral vector with down-regulated FABP5 was constructed to infect human immortalized keratinocytes (HaCaT) cells, and the transfection efficiency was examined. The HaCaT cells were divided into blank control group, FABP5 down-regulation group (FABP5), radiation group (IR), and FABP5 down-regulation combined with radiation group (FABP5+IR). After 6 MV X-ray radiation, cell proliferation viability was measured by CCK-8 assay, cell migration was detected by scratch assay, apoptosis was analyzed by flow cytometry, radiosensitivity was evaluated by cloning formation assay, and the cellular protein expressions of PARP1, γ-H2AX, AKT and p-AKT were detected by Western blot.Results FABP5 was successfully knocked-down in both RNA level (t=25.14, P<0.05) and protein level (t=20.06, P<0.05). The down-regulation of FABP5 decreased the abilities of cells proliferation (t=3.55, 5.88, 3.18, P<0.05) and migration (t=15.44, P<0.05), but increased cell resistance to irradiation with a radiosensitization ratio of 0.782. The apoptosis rate of FABP5+IR group was significantly lower than IR group (22.05±6.71)% vs. (9.82±1.45)%, t=3.08, P<0.05. The protein levels of PARP1 and γ-H2AX in FABP5+IR group were also lower than those in the IR group 0.04±0.04, 0.11±0.06, 0.26±0.11, 0.22±0.07, 0.21±0.10, 0.52±0.22, 0.57±0.06, 0.43±0.02(t=2.83, 3.07, 4.50, 5.33, P<0.05), while the protein level of p-Akt in FABP5+IR group was higher than that in IR group (t=-16.24—3.02, P<0.05).Conclusions Down-regulation of FABP5 inhibited cell proliferation and migration, increased radioresistance, and reduced radiation-induced apoptosis and DNA damage of skin cells probably through PI3K/AKT signaling pathway. |
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