| Tang Shaokai,Chen Jianhui,Geng Fenghao,et al.The radiosensitization effect of STING agonist on cutaneous melanoma cells[J].Chinese Journal of Radiological Medicine and Protection,2023,43(1):1-7 |
| The radiosensitization effect of STING agonist on cutaneous melanoma cells |
| Received:August 26, 2022 |
| DOI：10.3760/cma.j.cn112271-20220826-00350 |
| KeyWords:Cutaneous melanoma Stimulator of interferon gene Radiosensitivity |
| FundProject:国家自然科学基金(82073477);四川省杰出青年科技人才项目(2022JDJQ0051);四川大学-泸州科技创新研发项目(2021CDLZ-9) |
| Author Name | Affiliation | E-mail | | Tang Shaokai | Laboratory of Radiation Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China | | | Chen Jianhui | Laboratory of Radiation Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China | | | Geng Fenghao | Laboratory of Radiation Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China | | | Yang Tingyi | Laboratory of Radiation Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China | | | Li Xiaoqian | Laboratory of Radiation Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China | | | Du Xiaobo | NHC Key Laboratory of Nuclear Technology Medical Transformation, Mianyang Central Hospital, Mianyang 621099, China | | | Zhang Shuyu | Laboratory of Radiation Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China | zhang.shuyu@hotmail.com |
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| Abstract:: |
| Objective To investigate the radiosensitizing effect and underlying mechanism of STING agonist (c-di-AMP) on cutaneous melanoma cells.Methods Human cutaneous melanoma cells (A375) were divided into four groups: the control group, 10 μmol/L c-di-AMP group, X-ray irradiation group and X-ray irradiation combined with c-di-AMP group. The radiosensitizing effect of c-di-AMP on A375 cells was detected by CCK-8-based viability assay, lactate dehydrogenase (LDH) release assay, flow cytometry-based apoptosis assay, and colony formation assay. Western blot analysis was used to determine the expressions of cell death-related proteins.Results In combination with 10 Gy X-ray irradiation, 10 μmol/L c-di-AMP showed significant radiosensitization effect in A375 cells, which was evidenced by decreased cell activity (t=5.11, P<0.05), increased cytotoxicity (t=10.15, P<0.05) and cell apoptosis (t=4.41, P<0.05) and reduced clone viability(t=6.30, 3.55, 5.45, 3.55, P<0.05). The calculated radiosensitization ratio of c-di-AMP to A375 cells was 1.88. Moreover, 10 μmol/L c-di-AMP further increased the expressions of cell death-related proteins induced by radiation in A375 cells.Conclusions The STING agonist c-di-AMP can be used as a radiosensitizer for cutaneous melanoma, which may provide a novel strategy for radiotherapy. |
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