| Liao Lingli,Yang Fan,Ma Yuwei,Wang Luyao,Qu Zhen,Wang Xiaojing,Jiang Hao,Zhou Yongchun.Ionizing radiation promotes epithelial-mesenchymal transition of cervical cancer cell line Siha through increasing the secretion of exosomes[J].Chinese Journal of Radiological Medicine and Protection,2022,42(12):922-927 |
| Ionizing radiation promotes epithelial-mesenchymal transition of cervical cancer cell line Siha through increasing the secretion of exosomes |
| Received:September 08, 2022 |
| DOI：10.3760/cma.j.cn112271-20220908-00366 |
| KeyWords:Cervical cancer|Ionizing radiation|Epithelial-mesenchymal transition|Exosomes |
| FundProject:国家自然科学基金(31200633);安徽省自然科学基金面上项目(2008085MH245);蚌埠医学院"512人才培育计划"项目(BY51201211);蚌埠医学院研究生科研创新计划项目(Byycx21093) |
| Author Name | Affiliation | E-mail | | Liao Lingli | Department of Radiation Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China | | | Yang Fan | Department of Radiation Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China | | | Ma Yuwei | Department of Radiation Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China | | | Wang Luyao | Department of Pathology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China | | | Qu Zhen | Anhui Province Key Laboratory of Clinical and Preclinical Research in Respiratory Disease, Molecular Diagnosis Center, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China | | | Wang Xiaojing | Anhui Province Key Laboratory of Clinical and Preclinical Research in Respiratory Disease, Molecular Diagnosis Center, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China | | | Jiang Hao | Department of Radiation Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China | | | Zhou Yongchun | Department of Radiation Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China | zhouyongchun@bbmc.edu.cn |
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| Abstract:: |
| Objective To observe the occurrence of epithelial-mesenchymal transition (EMT) in cervical cancer cell line Siha irradiated by X-rays with clinical conventional fraction radiotherapy model and investigate the role of exosomes in this process.Methods Siha cells were irradiated by 6 MV-X rays with 50 Gy in 25 fractions. EMT was evaluated by cell morphology, EMT biomarkers and cell migration and invasion ability. Exosomes released from cells were detected by transmission electron microscopy and nanoparticle tracking analysis (NTA), and its function in EMT was explored by using an exosome inhibitor GW4869 (10 μmol/L).Results After irradiation, EMT phenomenon was induced in the survived Siha cells, including the incidence of mesenchymal phenotype, upregulation of epithelial marker E-cadherin (t=9.66, P<0.05), downregulation of mesenchymal marker N-cadherin (t=41.61, P<0.05), and increase of cell migration and invasion abilities (t=6.11, 13.22; P<0.05). Meanwhile, the secretion of exosomes was also increased after irradiation (t=7.51, P0.05). When the cells were pre-treated with GW4869, radiation-induced exosome secretion was reduced (t=7.28, P0.05), so that radiation-induced EMT was reversed.Conclusions Ionizing radiation with clinical conventional fraction radiotherapy model promotes EMT of cervical cancer cells through increasing the secretion of exosomes. |
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