| Liu Guoli,Song Xiujun,Wang Yemei,Yu Zuyin,Shen Xing,Shi Lei,Jin Hua,Wang Xinru.Preventive and therapeutic effects of Bateri-7 on radiation-induced intestinal injury in mice[J].Chinese Journal of Radiological Medicine and Protection,2022,42(11):839-844 |
| Preventive and therapeutic effects of Bateri-7 on radiation-induced intestinal injury in mice |
| Received:June 30, 2022 |
| DOI：10.3760/cma.j.cn112271-20220630-00275 |
| KeyWords:Mongolian medicine Bateri-7 Radiation-induced intestinal injury Radiation enteritis |
| FundProject:内蒙古民族大学科学研究项目(NMDYB20067) |
| Author Name | Affiliation | E-mail | | Liu Guoli | Department of Clinical Laboratory, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000, China | | | Song Xiujun | Department of Clinical Laboratory, PLA Rocket Army Characteristic Medical Center, Beijing 100088, China | | | Wang Yemei | Graduate Training Base of Jinzhou Medical University, PLA Rocket Army Characteristic Medical Center, Beijing 100088, China | | | Yu Zuyin | Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China | | | Shen Xing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China | | | Shi Lei | Department of Clinical Laboratory, PLA Rocket Army Characteristic Medical Center, Beijing 100088, China | | | Jin Hua | Department of Clinical Laboratory, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000, China | | | Wang Xinru | Department of Clinical Laboratory, PLA Rocket Army Characteristic Medical Center, Beijing 100088, China | wangxinru@126.com |
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| Abstract:: |
| Objective To study the protective effect of Mongolian medicine Bateri-7 on radiation-induced intestinal injury in mice.Methods C57BL/6J male mice were randomly divided into control group, irradiation group and irradiation plus drug administration group, with 10 or 15 mice in each group. For irradiation group, the mice were given a single dose of 12 Gy 60Co γ-rays with total body irradiation. For drug treatment, the mice were gavaged with Bateri-7 (530 mg/kg) 7 d before irradiation until 3 d after IR. At 6 h and 24 h after irradiation, the Tunel positive cells in intestine were detected immunohistochemically. At 3.5 d after irradiation, the structure of intestinal villi was observed by HE staining, and the BrdU and Ki67 positive cells were detected immunohistochemically. The expression levels of IL-6, TNF-α and Cxcl-5 were detected by qPCR. The FITC-dextran in peripheral blood was also determined.Results The survival of irradiated mice was significantly increased by Bateri-7 (χ2= 5.84, P < 0.05), but there was no significant difference in weight between two groups (P > 0.05). The villi length of small intestine in the irradiation plus drug group was significantly longer than that in the irradiation group (t = 20.24, P < 0.05), and there was no significant difference in the depth of intestinal crypt between two groups (P > 0.05). At 6 and 24 h after irradiation, the number of Tunel positive cells in intestinal crypts in the irradiation plus drug group was significantly reduced in comparison with the irradiation group (t = 3.52, 2.90, P < 0.05). At 3.5 d after irradiation, the level of FITC-dextran in serum and the expressions of IL-6, TNF-α and Cxcl-5 in small intestine of mice in the irradiation plus drug group were significantly lower than those in the irradiation group, respectively(t = 6.92, 7.01, 7.18, 13.16,P < 0.05). The number of BrdU and Ki67 positive cells in the crypt of mice in the irradiation plus drug group was higher than that of the irradiation group (t = 3.91, 2.57, P < 0.05).Conclusions Mongolian medicine Bateri-7 can effectively alleviate irradiation-induced intestinal injury of mice, which may have a good preventive and therapeutic effect on radiation enteritis. |
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