| Zhao Wendi,Tian Jian,Xuan Dandan,Zhang Xiuli,Li Chunmei,Zhou Jianwei.Prevention effect of low dose pre-irradiation on irradiation induced lung injury in mice and its possible mechanism[J].Chinese Journal of Radiological Medicine and Protection,2022,42(10):745-752 |
| Prevention effect of low dose pre-irradiation on irradiation induced lung injury in mice and its possible mechanism |
| Received:December 27, 2021 |
| DOI：10.3760/cma.j.cn112271-20211227-00498 |
| KeyWords:Ionizing radiation Low dose Prevention of radiation lung injury Pulmonary fibrosis |
| FundProject:国家自然科学基金 (81673097) |
| Author Name | Affiliation | E-mail | | Zhao Wendi | Henan University People's Hospital, Henan Provincial People's Hospital Cancer Center, Zhengzhou 450003, China | | | Tian Jian | Henan University People's Hospital, Henan Provincial People's Hospital Cancer Center, Zhengzhou 450003, China | | | Xuan Dandan | Experimental Animal Center of Zhengzhou University, Zhengzhou 450003, China | | | Zhang Xiuli | Henan University People's Hospital, Henan Provincial People's Hospital Cancer Center, Zhengzhou 450003, China | | | Li Chunmei | Henan University People's Hospital, Henan Provincial People's Hospital Cancer Center, Zhengzhou 450003, China | | | Zhou Jianwei | Henan University People's Hospital, Henan Provincial People's Hospital Cancer Center, Zhengzhou 450003, China | zhoujw666@163.com |
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| Abstract:: |
| Objective To evaluate the prevention effect of low dose pre-irradiation on irradiation-induced lung injury and its possible mechanism.Methods Totally 320 6-week-old female C57BL/6j mice were divided into control (0 Gy), low-dose (0.5 Gy), high-dose (20 Gy) and low-dose pre-radiation(0.5 Gy+20 Gy)groups by the random number method, with 80 mice in each group. The mice in the low-dose and low-dose pre-irradiation groups were placed in the immobilization device under full consciousness and subjected to 0.5 Gy X-ray whole-body irradiation. 2 weeks later, the 0.5 Gy pre-irradiated mice were anesthetized and subjected to 20 Gy X-rays on chest, as the pre-radiation plus high dose radiation group. The mice in the control group were irradiated with mock irradiation (0 Gy). All mice were terminated at designed time points (24 h, 1 month, 3 months and 5 months) after completion of the irradiation schedule, with 20 mice/group at each time point. Then, lung tissues were taken from mice, and pathological changes were observed by hematoxylin-eosin (HE) staining and Masson's trichrome staining. RT-qPCR and Western blot were used to detect the expressions of mRNAs and proteins of pulmonary fibrosis-related factors.Results Pathological changes were observed in the lung tissues 1 month after a single high-dose 20 Gy irradiation, mainly including radiation pneumonitis and a small amount of collagen accumulation, which was more serious than low-dose pre-irradiation group, and these pathological changes became more severe when the time after irradiation increased. Meanwhile, the mRNA and protein levels of proSP-C and HOPX in the low-dose pre-irradiation group were higher than those in the high-dose group, except for proSP-C protein expression at 3 and 5 months post-irradiation. A more significant change was that the mRNA level of TGF-β1 in the high-dose group was 5.8-13.6 times higher than that in the other groups at 5 months after irradiation, as well as β-catenin mRNA (t=4.22, 5.11, P<0.05). At the same time, in the early period (24 h and 1 month) post-irradiation, the level of vimentin protein in the low-dose pre-irradiation group was significantly higher than that in the high-dose group (t=6.54, 4.28, P<0.05).Conclusions When the mice were pre-irradiated with 0.5 Gy X-rays, an adaptive protective response was induced in lung tissues, resulting in the tolerance to subsequent high dose irradiation. |
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