| Zhu Tong,He Junbo,Wu Xin,et al.Mechanism of Sedum alfredii extract alleviating radiation damage in human small intestinal epithelial cells HIEC-6[J].Chinese Journal of Radiological Medicine and Protection,2022,42(7):493-498 |
| Mechanism of Sedum alfredii extract alleviating radiation damage in human small intestinal epithelial cells HIEC-6 |
| Received:January 04, 2022 |
| DOI：10.3760/cma.j.cn112271-20220104-00002 |
| KeyWords:Sedum alfredi extract Radiation damage PRKN Endoplasmic reticulum ROS |
| FundProject:国家自然科学基金(81730086);中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-042);中央高校基本科研业务费专项资金(3332019098) |
| Author Name | Affiliation | E-mail | | Zhu Tong | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | He Junbo | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | Wu Xin | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | Wang Zhouxuan | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | Fan Saijun | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | fansaijun@irm-cams.ac.cn |
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| Abstract:: |
| Objective To confirm the mechanism of Sedum alfredii extract (SafE) alleviating radiation injury in human small intestinal epithelial cells (HIEC-6). Methods HIEC-6 cells were divided into 4 groups, including control group (Con), irradiation group (IR), SafE alone group (SafE) and SafE plus irradiation group (SafE+IR). All of the SafE groups were treated with 0.02 g/ml (W/V) SafE for 24 h. Cell viability (CCK-8 method ) and intracellular ROS levels were investigated at 24 h after 2, 4, and 6 Gy irradiation. Samples were taken at 24 h after 4 Gy irradiation for transcriptome analysis, and the intracellular E3 ubiquitin ligase PRKN expression level was measured. The thickness of endoplasmic reticulum was detected at 24 h after 4 Gy irradiation using fluorescent dye. Results SafE could maintain cell viability after irradiation (t=2.94-10.40, P＜0.05), and significantly reduced the level of ROS in the irradiated cells (t=-13.29--4.53, P＜0.05). PRKN was preliminarily verified to be the target gene of SafE that maintained PRKN transcript level and endoplasmic reticulum thickness after irradiation (IR group vs. Con group: t=-5.55, 3.27, P<0.05, SafE group vs. SafE+IR group: P>0.05).Conclusion SafE is effective in maintaining ER thickness and reducing cellular radiation damage and its target gene PRKN could be regulated by ionizing radiation. |
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