| Hu Cuicui,Chang Chenxu,Kong Xiaoxu,et al.Influence of microwave radiation on associative learning and memory function and hippocampal structure in mice[J].Chinese Journal of Radiological Medicine and Protection,2022,42(7):481-486 |
| Influence of microwave radiation on associative learning and memory function and hippocampal structure in mice |
| Received:March 24, 2022 |
| DOI：10.3760/cma.j.cn112271-20220324-00120 |
| KeyWords:Microwave radiation Mouse Hippocampus Learning and memory Tissue structure |
| FundProject:北京市自然科学基金面上项目(7222250) |
| Author Name | Affiliation | E-mail | | Hu Cuicui | Academy of Life Sciences, Anhui Medical University, Hefei 230032, China | | | Chang Chenxu | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Kong Xiaoxu | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Liu Yadong | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Ye Yumeng | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Hao Yanhui | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Zuo Hongyan | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Li Yang | Academy of Life Sciences, Anhui Medical University, Hefei 230032, China | leeyoung33@163.com |
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| Abstract:: |
| Objective To investigate the effects of microwave radiation on associative learning and memory function and hippocampal structure of mice. Methods C57BL/6N mice were ramdomly divided into sham-radiated group (n=27) and radiation group (n=2). The radiation group was exposed to microwave at 2.856 GHz, 8 mW/cm2 for 15 min, then their spatial and associative learning and memory function were examined with the morris water maze and shuttle box behavior experiment. The pathological changes of hippocampal tissue were observed by HE staining and light microscope, the ultrastructural changes of hippocampal tissue were observed by transmission electron microscope. Results After microwave radiation, the times of mice crossing the platform for the reverse space exploration decreased from(3.60±0.79) times to (2.55±0.47) times(t=2.21, P=0.046), the average active escape rate decreased significantly (t = 2.70, P<0.05), and the average active latency and the total shock time was significantly prolonged (t = -3.09, -3.02, P < 0.05). At 8 d after microwave radiation, the nuclei of some neurons in the CA3 and DG regions of the hippocampus were pyknosis. The neurons were apoptotic, the synaptic spaces blurred, the glial cells swollen, and the perivascular spaces widened in the CA3 region of the hippocampus. Conclusions Microwave radiation can decline the spatial reference memory ability and associative learning and memory ability of mice. The morphological and pathological changes of hippocampus are the structural basis of this dysfunction. |
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