| Chen Zhongmin,Zhang Meng,Xu Longjiang,Li Fengsheng,Sun Liang,Chen Qiu,Tu Yu,Cui Fengmei.Screening and preliminaryfunctional analysis of differentially expressed lncRNAs and mRNAs in vascular endothelial cells treated by tritiated water[J].Chinese Journal of Radiological Medicine and Protection,2022,42(6):428-432 |
| Screening and preliminaryfunctional analysis of differentially expressed lncRNAs and mRNAs in vascular endothelial cells treated by tritiated water |
| Received:January 11, 2022 |
| DOI：10.3760/cma.j.cn112271-20220111-00014 |
| KeyWords:Tritiated water Vascular endothelial cells lncRNAs mRNAs |
| FundProject:国家自然科学基金核技术创新联合基金(U186720);军队后勤科研计划(BEP20J004) |
| Author Name | Affiliation | E-mail | | Chen Zhongmin | PLA Rocket Characteristic Medical Center, Beijing 100088, China | | | Zhang Meng | The Health Supervision Institute of Lianyungang, Lianyungang 222002, China | | | Xu Longjiang | State Key Laboratory of Radiation Medicine and Protection, Soochou University, Suzhou 215123, China | | | Li Fengsheng | PLA Rocket Characteristic Medical Center, Beijing 100088, China | | | Sun Liang | State Key Laboratory of Radiation Medicine and Protection, Soochou University, Suzhou 215123, China | | | Chen Qiu | State Key Laboratory of Radiation Medicine and Protection, Soochou University, Suzhou 215123, China | | | Tu Yu | State Key Laboratory of Radiation Medicine and Protection, Soochou University, Suzhou 215123, China | | | Cui Fengmei | State Key Laboratory of Radiation Medicine and Protection, Soochou University, Suzhou 215123, China | cuifengmei@suda.edu.cn |
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| Abstract:: |
| Objective To investigate the expression changes of lncRNAs and mRNAs in human umbilical vein endothelial cells(HUVEC) treated by tritiated water.Methods HUVEC cells were divided into two groups, the control group cultured in DMEM medium, and the tritiated water exposure group cultured in a medium containing tritiated water with a final concentraion of 3.7×103 Bq/ml. After culture for 48 h, cells were collected for RNA extract.The differentially expressed lncRNAs and mRNAs were screened by high-through put chip technology and then analyzed.Results Compared with the control group, 1 717 lncRNAs were significantly up-regulated and 3 994 lncRNAs significantly down-regulated, and 4 562 mRNAs were significantly up-regulated and 1 433 mRNAs down-regulated. Through co-expression analysis of differential mRNAs and lncRNAs, some key genes including SQSTM1, CXCL8, ITPR1, GADD45A, NF-kB1 and VDAC1 were obtained.Conclusions Tritiated water exposure can induce multiple changes of mRNAs and lncRNAs in vascular endothelial cells, which may lead to toxic effects through signaling pathways including some key genes such as SQSTM1, CXCL8, and ITPR1. |
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