| Wang Qi,Zhao Ke,Gao Yameng,et al.Effects of ionizing radiation on mitochondrial function of mouse hematopoietic stem and progenitor cells[J].Chinese Journal of Radiological Medicine and Protection,2022,42(5):321-327 |
| Effects of ionizing radiation on mitochondrial function of mouse hematopoietic stem and progenitor cells |
| Received:February 22, 2022 |
| DOI：10.3760/cma.j.cn112271-20220222-00064 |
| KeyWords:Hematopoietic stem and progenitor cells Mitochondria Ionizing radiation |
| FundProject:国家自然科学基金面上项目(81770108);蛋白质组国家重点实验室自主课题(SKLP-K201802) |
| Author Name | Affiliation | E-mail | | Wang Qi | School of Basic Medicine, Auhui Medical University, Hefei 230032, China | | | Zhao Ke | Beijing Institute of Lifeomics, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China | | | Gao Yameng | School of Basic Medicine, Auhui Medical University, Hefei 230032, China | | | Li Xin | The Fifth Medical Center, General Hospital of PLA, Beijing 100071, China | | | Wu Yunqiang | Beijing Institute of Lifeomics, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China | | | Zhu Yaxin | School of Life Sciences, Hebei University, Baoding 071028, China | | | Lyu Zhichun | Beijing Institute of Lifeomics, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China | | | Sun Huiying | Beijing Institute of Lifeomics, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China | | | Gao Huiying | Beijing Institute of Lifeomics, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China | | | Xiang Shensi | Beijing Institute of Lifeomics, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China | | | Li Changyan | School of Basic Medicine, Auhui Medical University, Hefei 230032, China | fmmli@163.com |
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| Abstract:: |
| Objective To study the effect of different doses of 60Co γ-ray ionizing radiation on mitochondrial function in mouse hematopoietic stem and progenitor cells (HSPCs).Methods C57BL/6 mice were divided into control group, 1 Gy irradiation group and 4.5 Gy irradiation group. The mitochondrial functions were detected at 12 h and 24 h after irradiation, including ROS level, membrane potential, mitochondrial structure, and mitochondrial stress. Bone marrow c-Kit+ cells received a single 15 Gy irradiation in vitro, after 24 h, mitochondrial function was detected.Results It was found that mice leukocytes (t=12.41, 18.31, 16.48, 14.16, 19.08, 20.25, P<0.05), red blood cells (t=4.81, 6.62, P<0.05) and platelets (t=4.33, 6.68, P<0.05) were significantly reduced. The numbers of bone marrow colony formation unit (t=16.27, 55.66, 17.06, 43.75, P<0.05), and HSPCs (t=5.16, 11.55, P<0.05) were decreased dose-dependently post-irradiation. Under 1 Gy irradiation, the mitochondrial function and mitochondrial basal metabolic index of HSPCs (t= 7.36, 3.68, 4.58, 3.15, 3.15, P<0.05) were enhanced at 24 h post-irradiation. Under 4.5 Gy irradiation, mitochondrial number, mitochondrial membrane potential (t=12.29, 10.46, P<0.05), maximal respiration and spare respiratory capacity were decreased (t=7.81, 5.78, 6.70, 5.83, P<0.05), ROS level was increased (t=4.63, 4.12, P<0.05). The basal respiration and oxidative phosphorylated ATP production were reduced at 12 h after irradiation (t=8.48, 3.80, P<0.05); and the proton leakage was increased (t=6.57, P<0.05) and coupling efficiency was reduced (t=11.43, P<0.05) at 24 h after irradiation. In cultured c-Kit+ cells, the level of ROS (t=11.30, P<0.05) and the maximum respiration and spare respiratory capacity were increased (t=4.25, 3.44, P<0.05) while the mitochondrial membrane potential was decreased (t=34.92, P<0.05) significantly.Conclusions A method for systematically assessing mitochondrial function in HSPCs was established, and the effect of ionizing radiation on mitochondrial function of HSPCs was clarified, laying a foundation for further revealing the mechanism of ionizing radiation-induced mitochondrial damage in HSPCs. |
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