| Li Changyong,Jia Meng,Wang qi,Yan Linjun,Wang Zhidong,Qi Zhenhua.MiR-375-3p enhanced radiosensitivity of colorectal cancer cells by inhibiting homologous recombination repair of DSBs[J].Chinese Journal of Radiological Medicine and Protection,2022,42(3):168-174 |
| MiR-375-3p enhanced radiosensitivity of colorectal cancer cells by inhibiting homologous recombination repair of DSBs |
| Received:October 27, 2021 |
| DOI：10.3760/cma.j.cn112271-20211027-00425 |
| KeyWords:Colorectal cancer miR-375-3p Homologous recombination repair RAD51 Radiosensitivity |
| FundProject: |
| Author Name | Affiliation | E-mail | | Li Changyong | Institute of Radiation Medicine, Institute of Military Medical Research, Beijing Key Laboratory of Radiobiology, Beijing 100850, China | | | Jia Meng | Institute of Radiation Medicine, Institute of Military Medical Research, Beijing Key Laboratory of Radiobiology, Beijing 100850, China | | | Wang qi | Institute of Radiation Medicine, Institute of Military Medical Research, Beijing Key Laboratory of Radiobiology, Beijing 100850, China | | | Yan Linjun | Jing Xi Medical District PLA Genera Hospital, Beijing 100144, China | | | Wang Zhidong | Institute of Radiation Medicine, Institute of Military Medical Research, Beijing Key Laboratory of Radiobiology, Beijing 100850, China | | | Qi Zhenhua | Institute of Radiation Medicine, Institute of Military Medical Research, Beijing Key Laboratory of Radiobiology, Beijing 100850, China | tjuqzh@163.com |
|
| Hits: 2143 |
| Download times: 634 |
| Abstract:: |
| Objective To investigate the effect of miR-375-3p on DNA damage repair and radioresistance of colorectal cancer cells. Methods After overexpression of miR-375-3p in HCT116 and HT29, cell proliferation ability was detected by CCK-8 assay, clone formation ability was detected by clone formation assay, apoptosis was detected by Annexin V-FITC/PI double staining method and cell cycle distribution was detected by flow cytometry, and the formation of γ-H2AX foci were used to analyze homologous recombination (HR) repair efficiency. Bioinformatics was used to predict the downstream target genes of miR-375-3p in the HR repair pathway. A dual luciferase reporter gene assay was used to validate the regulation effect of miR-375-3p on Rad51 gene. The expression of miR-375-3p in HCT116 cells irradiated with 60Co γ-rays at 2 and 6 Gy was measured by RT-qPCR. The inhibition effect of miR-375-3p on the radiosensitivity of HCT116 cells was analyzed after irradiation with different doses of 0, 1, 2, 4 and 6 Gy. Results Overexpression of miR-375-3p inhibited the proliferation and colony formation ability, induced G1 phase cycle arrest and cell apoptosis of colorectal cancer cells, enhanced DSBs formation, inhibited Rad51 expression, and significantly decreased HR repair efficiency (t=10.055, P<0.05). Dual luciferase reporter gene assay demonstrated that miR-375-3p bound to Rad51 3'UTR region (t=5.013, P<0.05). In addition, irradiation increased miR-375-3p expression, and inhibition of miR-375-3p expression reduced radiosensitivity of colorectal cancer cells (t=6.460, 5.619,10.150, P<0.05). miR-375-3p inhibited the homologous recombination repair efficiency of DSBs and enhanced the radiosensitivity of colorectal cancer cells. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
