| He Gaiying,Gou Wenfeng,Wang Yi,Hou Wenbin,Tang Jinghui,Ma Shuhua,Sun Yanan,Yang Weifeng,Fang Qiang,Li Yiliang.Application of two-photon imaging technology in the repair evaluation of radiation-induced skin injury in rats[J].Chinese Journal of Radiological Medicine and Protection,2021,41(12):926-930 |
| Application of two-photon imaging technology in the repair evaluation of radiation-induced skin injury in rats |
| Received:August 03, 2021 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.12.008 |
| KeyWords:Radiation skin injury Two-photon excitation fluorescence imaging Epidermal cells Collagen fibers Damage repair |
| FundProject:北京自然科学基金面上项目（7192141） |
| Author Name | Affiliation | E-mail | | He Gaiying | Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China | | | Gou Wenfeng | Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Tianjin 300192, China | | | Wang Yi | Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China | | | Hou Wenbin | Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Tianjin 300192, China | | | Tang Jinghui | College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China | | | Ma Shuhua | Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China | | | Sun Yanan | Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China | | | Yang Weifeng | Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China | | | Fang Qiang | Civil Aviation Medical Center of CAAC Northeast Regional Administration, Shenyang 110066, China | | | Li Yiliang | Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Tianjin 300192, China | liyiliang@irm-cams.ac.cn |
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| Abstract:: |
| Objective To evaluate the skin development and repair process of X-ray radiation damage in rat with non-invasive two-photon excitation fluorescence (TPEF) imaging technology in vivo. Methods Totally 24 SD rats were randomly divided into four groups including X-ray irradiated group (25, 35 and 45 Gy) and non-irradiation control group. At different times after irradiation, the degree of skin injury was evaluated, and the pathological changes of nicotinamide adenine dinucleotide (phosphate)[NAD(P)H] and collagen fiber fluorescence signals in epidermal cells were detected in vivo by TPEF imaging technology. Results At 10 d post-irradiation, the skin of irradiation groups showed erythema and desquamation. At 15-20 d post-irradiation, the skin of radiation groups developed progressive exudation, edema and ulcers with increasing radiation dose. On day 25, the skin began to repair in the 25 Gy group, however, the skin of other groups still had exudation and ulcers. On day 10, NAD(P)H fluorescence signal in epidermal cells of irradiation groups decreased and the fluorescence signal of collagen fibers in papillary layer and reticular layer of irradiation groups reduced, which were significantly lower than that of normal control group (t=24.145, 28.303, 26.989, 6.654, 7.510, 7.997, P<0.05). On day 30, fluorescence signal of NAD(P)H and collagen fibers in epidermal cells and dermis began to repair, the cell from stratum granulosum, stratum spinosum, and stratum basale in the 25 Gy group showed fluorescence signal, the other groups did not show. The fluorescence signal of collagen fibers in the 25 Gy group were gradually increased in papillary layer and reticular layer, however, they were significantly lower than normal control group (t=115.133, 17.431, P<0.05), the skin of 45 Gy group did not show fluorescence signal of collagen fibers. Conclusions The damage and repair process of epidermal cells and dermal collagen fiber can be detected noninvasively by TPEF imaging technology after X-ray irradiation in vivo. |
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