| Ma Liping,Liu Qingjie,Tian Yuting,Liu Fang,Tian Mei,Gao Ling.Melatonin inhibits UVB-induced melanin synthesis by regulating p53-mediated premature senescence in HaCaT cells[J].Chinese Journal of Radiological Medicine and Protection,2021,41(12):920-925 |
| Melatonin inhibits UVB-induced melanin synthesis by regulating p53-mediated premature senescence in HaCaT cells |
| Received:July 20, 2021 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.12.007 |
| KeyWords:Melatonin Melanin synthesis Premature senescence p53 TYR |
| FundProject:北京市自然科学基金（7162139，7202137）；国家自然科学基金（31570852） |
| Author Name | Affiliation | E-mail | | Ma Liping | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Yuting | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Fang | Department of Dermatology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China | | | Tian Mei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Gao Ling | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | gaolingxinxin@126.com |
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| Abstract:: |
| Objective To explore the mechanism and regulatory effects of melatonin on UVB-induced melanin synthesis in human immortalized keratinocytes (HaCaT), so as to provide a theoretical basis for the skin protection of melatonin. Methods HaCaT cells were pretreated with 10-5 mol/L melatonin and then irradiated with 80 mJ/cm2UVB. The melanin content was detected by NaOH assay, the proportion of premature senescence cells was detected by β-galactosidase staining kit, and the protein expression levels of both p53 and tyrosinase (TYR) were detected by Western blot at 72 h after UVB exposure. After 12 h pretreatment of ATM/ATR inhibitor, p53 inhibitor and melatonin, the proportion of premature senescence and the change of melanin content in HaCaT cells were detected at 72 h after 80 mJ/cm2 UVB irradiation. Results Melatonin inhibited UVB-induced increases of melanin content (t=56.65, 13.39, P<0.05) and TYR expression (t=16.46, P<0.05) in HaCaT cells. Melatonin alleviated UVB-induced premature senescence (t=7.139, P<0.05) and inhibited UVB-induced increase of p53 expression (t=19.08, P<0.05) in HaCaT cells. In addition, ATM/ATR inhibitor, p53 inhibitor and melatonin all inhibited UVB-induced increase of melanin content in HaCaT cells. Conclusions Melatonin inhibits TYR-mediated melanin synthesis by regulating p53-related premature senescence in HaCaT cells after UVB irradiation. |
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