| Yang Chongshuang,Liu Pingping,Huang Xuequan,Xiao Yunhua,Liu Henan,Li Liangshan,Li Tingyuan.Experimental study of 125I seeds inhibiting angiogenesis of transplanted hepatocellular carcinoma[J].Chinese Journal of Radiological Medicine and Protection,2021,41(11):808-812 |
| Experimental study of 125I seeds inhibiting angiogenesis of transplanted hepatocellular carcinoma |
| Received:May 08, 2021 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.11.002 |
| KeyWords:Hepatocellular carcinoma 125I seeds VEGF-A HIF-1α |
| FundProject:重庆市社会事业与民生保障科技创新专项（cstc-2016shms-ztzx0045） |
| Author Name | Affiliation | E-mail | | Yang Chongshuang | Department of Nuclear Medicine, the First Affiliated Hospital of Army Medical University, Chongqing 400038, China | | | Liu Pingping | Department of Nuclear Medicine, the First Affiliated Hospital of Army Medical University, Chongqing 400038, China | | | Huang Xuequan | Department of Nuclear Medicine, the First Affiliated Hospital of Army Medical University, Chongqing 400038, China | hxuequan@163.com | | Xiao Yunhua | Department of Nuclear Medicine, the First Affiliated Hospital of Army Medical University, Chongqing 400038, China | | | Liu Henan | Department of Nuclear Medicine, the First Affiliated Hospital of Army Medical University, Chongqing 400038, China | | | Li Liangshan | Department of Nuclear Medicine, the First Affiliated Hospital of Army Medical University, Chongqing 400038, China | | | Li Tingyuan | Department of Nuclear Medicine, the First Affiliated Hospital of Army Medical University, Chongqing 400038, China | |
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| Abstract:: |
| Objective To investigate the effect of radioactive 125I seed on angiogenesis of subcutaneously transplanted hepatocellular carcinoma in nude mice and underlying mechanism. Methods The subcutaneous transplanted tumor model of human hepatocellular carcinoma Huh7 cells was established in nude mice. Twelve nude mice were randomly divided into observation group and control group with 6 mice in each group. The 125I seed with activity of 2.96×107 Bq was implanted into the transplanted tumor of observation group and another with 0 Bq as control group, respectively. The volume of the transplanted tumor was measured every 4 d and the growth curve of the tumor was recorded. The microvessel density (MVD) of the transplanted tumor was evaluated by immunohistochemical detection of CD31. VEGF-A and HIF-1α protein and mRNA were detected by immunohistochemistry and RT-PCR, respectively. Results The growth rate of tumor in the observation group was slower than that in the control group, and the difference of tumor volume between two groups at 12 d after 125I seed implantation was significantly different(t=3.167, P<0.05). At 28 d after transplantation, the tumor volumes of control and observation group approached to (963.61 ±89.56) mm3 and (602.10±75.98) mm3, respectively. The MVD of the observation group was significantly lower than that of the control group (t=6.361, P<0.05). The relative expression of VEGF-A and HIF-1α mRNA in the observation group was significantly lower than that in the control group (t=10.480, 6.414, P<0.05). Protein expression levels of VEGF-A and HIF-1α in the observation group were lower than those in the control group (t=10.890, 12.250, P<0.05). Conclusions Radioactive 125I seed can inhibit the growth of HCC xenografts by reducing tumor microvessels, which may be related to the decrease of VEGF-A and HIF-1α expression. |
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