| Wang Ning,Qu Donghao,Lin Yuhan,et al.Protective effect of α-lipoic acid on cochlear ribbon synapses in mice injured by ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2021,41(8):609-614 |
| Protective effect of α-lipoic acid on cochlear ribbon synapses in mice injured by ionizing radiation |
| Received:January 17, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.08.008 |
| KeyWords:Alpha-lipoic acid Ionizing radiation Ribbon synapses Cochlear |
| FundProject:辽宁省自然科学基金（2015020342） |
| Author Name | Affiliation | E-mail | | Wang Ning | Department of Otorhinolaryngology, First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, China | | | Qu Donghao | School of Basic Medicine, Jinzhou Medical University, Jinzhou 121000, China | | | Lin Yuhan | School of Basic Medicine, Jinzhou Medical University, Jinzhou 121000, China | | | Xu Tao | School of Life Sciences, Jinzhou Medical University, Jinzhou 121000, China | | | Liu Shuangyue | School of Basic Medicine, Jinzhou Medical University, Jinzhou 121000, China | | | Xing Weiwei | Department of Otorhinolaryngology, First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, China | lnxingweiwei@163.com |
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| Abstract:: |
| Objective To explore the protective effect of α-lipoic acid (LA) on radiation damage of mice cochlear ribbon synapses.Methods Mice were divided into five groups:control group, radiation 3 d group, radiation 3 d+LA group, radiation 14 d group and radiation 14 d+LA group. The radiation groups were irradiated with 16 Gy, the radiation+LA groups were given LA once a day after radiation, the control group was given the same amount of normal saline. The auditory brainstem response (ABR) of mice were measured before irradiation and sacrifice. The number of ribbon synapses were observed with immunofluorescently labeled protein ctBP2. Western blot assay was performed to obtain the semi-quantitative expression levels of otoferlin and AP-2 protein.Results Compared with the control group, the ABR threshold of radiation groups were significantly higher (P<0.05) with the highest value at 14 d after irradiation (P<0.05), and the ABR threshold of the radiation+LA groups were significantly lower (P<0.05). The ABR threshold shifts of 12 kHz, 24 kHz at 3 d and 14 d groups had no significant difference with 8 kHz threshold shift (P>0.05). The 32 kHz threshold shift was significantly higher than 8 kHz threshold shift (t=-2.38, -5.48, P<0.05). The number of ribbon synapses in the radiation groups was significantly lower than that of control group (P<0.05), with the lowest value in the radiation 14 d group. LA treatment increased the ABR value significantly (P<0.05). AP-2 and otoferlin protein levels were significantly reduced after irradiation, especially in the radiation 14 d groups, and they were increased by the LA treatment.Conclusions LA has protective effect on the ribbon synapses of cochlear hair cells. |
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