| Lu Lu,Zhang Junling,Li Deguan,et al.Metabonomic study on the effect of fasting on 137Cs γ-ray radiation-induced intestinal injury in mice[J].Chinese Journal of Radiological Medicine and Protection,2021,41(8):564-570 |
| Metabonomic study on the effect of fasting on 137Cs γ-ray radiation-induced intestinal injury in mice |
| Received:November 11, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.08.002 |
| KeyWords:Fasting Radiation injury Metabolomics |
| FundProject:国家自然科学基金面上项目（81703169，81572969，81730086）；天津市科技支撑计划项目（14ZCZDSY00001） |
| Author Name | Affiliation | E-mail | | Lu Lu | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical Collage, Tianjin 300192, China | | | Zhang Junling | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical Collage, Tianjin 300192, China | | | Li Deguan | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical Collage, Tianjin 300192, China | | | Fan Saijun | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical Collage, Tianjin 300192, China | fansaijun@irm-cams.ac.cn |
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| Abstract:: |
| Objective To study the effect of fasting on 137Cs γ-ray radiation-induced intestinal injury in mice, and to explore the effect of fasting on fecal metabolites of mice through non-targeted metabolomics.Methods C57BL/6 mice were divided into healthy control group, 9 Gy γ-ray whole body irradiation (WBI)/15 Gy γ-ray whole abdominal irradiation (WAI) group, fasting (24 h, 48 h, 72 h)+ 9 Gy WBI/15 Gy WAI group. After irradiation, the survival rate, spleen index and thymus index were calculated. C57BL/6 mice in non-target metabolism experiment were randomly divided into four groups:control group, fasting 24 h group, 15 Gy γ-ray WAI group, fasting 24 h + 15 Gy γ-ray WAI group, 6 mice in each group. After 15 Gy WAI, the feces of mice in each group were collected at 3.5 days for non-targeted metabolomics detection.Results The median survival time of mice with 48 h and 24 h fasting before 9 Gy γ-ray irradiation was increased by 1 day and 4 days, and the survival rates of mice treated with 48 h and 24 h fasting before 15 Gy WAI were 16.67% and 25%, respectively. 15 Gy γ-ray WAI on mice with fasting for 24 h before irradiation could increase the body weight (t=2.338, P=0.042) and spleen index (t=2.289, P=0.045) at 3.5 days after irradiation. Through non-targeted metabonomic analysis, it was found that there were 30 differentially expressed metabolites in fecal samples of fasting and non-fasting mice subjected to WAI, and metabolic pathway enrichment analysis showed that there was an imbalance in the metabolic pathway of steroid biosynthesis.Conclusions Fasting before irradiation can improve the survival rate of mice with intestinal radiation injury and change their intestinal metabolites, suggesting that pre-irradiation fasting or short-term dietary nutrition changes are involved in the regulation of intestinal radiation damage. |
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