| Li Shuang,Lu Xue,Cai Tianjing,Tian Mei,Liu Qingjie.Dose-rate effect on radiation-induced gene expressions in human peripheral blood[J].Chinese Journal of Radiological Medicine and Protection,2021,41(7):481-485 |
| Dose-rate effect on radiation-induced gene expressions in human peripheral blood |
| Received:January 26, 2021 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.07.001 |
| KeyWords:Ionizing radiation Gene expression Dose-rate |
| FundProject:国家自然科学基金（81803162） |
| Author Name | Affiliation | E-mail | | Li Shuang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Cai Tianjing | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Mei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | liuqingjie@nirp.chinacdc.cn |
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| Abstract:: |
| Objective To explore the influence of dose-rate on radiation-induced gene expression in human peripheral blood. Methods Human peripheral blood ex vivo was irradiated with 0, 1, 2, 4 and 6 Gy of 60Co γ-rays with different dose-rates of 0.2, 1 and 2 Gy/min. Human blood cells were harvested at 24 h post-irradiation. Following RNA isolation, the mRNA expression levels ofCDKN1A, MDM2, PCNA, FDXR, GADD45A, PHPT1, ASTN2, TNFSF4, POLH, GDF-15 and PPM1D were measured by quantitative real-time PCR. The stepwise regression method was used to establish the gene combination models. Results The relative mRNA expression levels of 11 genes significantly increased in a dose-dependent manner within the dose range of 0-6 Gy with three dose-rates of irradiation (R2=0.744-0.998, P< 0.05). Following the exposure to 2 Gy(0.2 Gy/min)60Co γ-rays, the expression levels of CDKN1A,FDXR,PHPT1 and TNFSF4 genes were significantly higher than that of the 1 and 2 Gy/min groups (t=3.73, 5.73, 2.44, 2.77, 3.53, 2.68, 2.43, 2.05, P< 0.05). With 6 Gy irradiation, the changes of radiation-induced PPM1D expression level under a dose rate of 2 Gy/min were significantly higher than other two dose-rates(t=3.82, 2.54, P< 0.05). The combined expression model at different dose rates was composed of 2-3 genes, and the R2values of regression equations were 0.976, 0.964 and 0.951, respectively. Conclusions In a certain range, the dose-rate may affect the changes of radiation-induced gene expression in human peripheral blood. |
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