| Li Tianyuan,Hai Long,Zhao Pengfei,Lu Xiaoxu,Sun Xueming,Huang Rong,Wu Hui.Effect of apatinib on radiosensitivity of glioma cells[J].Chinese Journal of Radiological Medicine and Protection,2021,41(4):253-258 |
| Effect of apatinib on radiosensitivity of glioma cells |
| Received:November 11, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.04.003 |
| KeyWords:Glioma cells Radiosensitivity Apatinib Apoptosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | Li Tianyuan | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Hai Long | Intelligent Pathology Institute, The First Affiliated Hospital of USTC, Hefei 230000, China | | | Zhao Pengfei | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Lu Xiaoxu | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Sun Xueming | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Huang Rong | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | | | Wu Hui | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China | Wuhui7008@126.com |
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| Abstract:: |
| Objective To investigate the effect of apatinib on radiosensitivity of glioma cells U87MG and its potential mechanism. Methods U87MG cells were divided into control group, apatinib group, radiation group and combination group treated with apatinib and radiation. The effect of different concentrations of apatinib (5, 10, 20, 40, 80 μmol/L) on cell proliferation was detected by CCK8 assay. The effect of apatinib on cell migration and invasion was detected by wound-healing assay and transwell assay, respectively. The effect of apatinib on cell radiosensitivity was detected by plate cloning assay, the cell apoptosis rate was detected by flow cytometry, and the protein expressions of Bax and Bcl-2 were detected by Western blot. Results Apatinib significantly inhibited the proliferation of U87MG cells in a manner depended on the drug treatment time and radiation. Compared with the radiation group, the cell proliferation, migration and invasion in the combination group were inhibited much significantly (t=9.857, 18.704, 4.197, P<0.05), so that the value of D0, Dq and SF2 of the combination group was lower, resulting in a radiosensitivity enhancement ratio (SERD0) of 1.3. Moreover, compared with the radiation group, the apoptosis rate of the combination group was increased, the expression of Bcl-2 protein was decreased, and the expression of Bax protein was increased (t=16.187, 8.890, 5.222, P<0.05). Conclusions Apatinib inhibits cell proliferation, invasion and migration, induces apoptosis and increases radiosensitivity of glioma cells. |
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