| Yu Daojiang,Tu Wenling,Jiang Zhiqiang,Wang Min,Shi Yuhong,Liu Yulong,Wang Youyou,Zhang Shuyu.Whole-genome sequencing analysis of the victim exposed to a super high dose of iridium-192 during the Nanjing radiological accident in 2014[J].Chinese Journal of Radiological Medicine and Protection,2021,41(4):247-252 |
| Whole-genome sequencing analysis of the victim exposed to a super high dose of iridium-192 during the Nanjing radiological accident in 2014 |
| Received:July 10, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.04.002 |
| KeyWords:Radiation accident Whole-genome sequencing Genetic variation |
| FundProject:国家自然科学基金（32071238，U1967220，31770911）；中国核工业集团青年英才计划项目（2018-272，2019-344）；四川省卫健委科研课题（20PJ227，20YYJC035） |
| Author Name | Affiliation | E-mail | | Yu Daojiang | Department of Plastic and Burn Surgery, Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation No. 416 Hospital, Chengdu 610051, China | | | Tu Wenling | Department of Plastic and Burn Surgery, Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation No. 416 Hospital, Chengdu 610051, China | | | Jiang Zhiqiang | Department of Plastic and Burn Surgery, Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation No. 416 Hospital, Chengdu 610051, China | | | Wang Min | Department of Plastic and Burn Surgery, Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation No. 416 Hospital, Chengdu 610051, China | | | Shi Yuhong | Department of Plastic and Burn Surgery, Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation No. 416 Hospital, Chengdu 610051, China | | | Liu Yulong | Department of Nuclear and Radiation Incident Medical Emergency Office, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Wang Youyou | Department of Nuclear and Radiation Incident Medical Emergency Office, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Zhang Shuyu | Department of Plastic and Burn Surgery, Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation No. 416 Hospital, Chengdu 610051, China | zhang.shuyu@hotmail.com |
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| Abstract:: |
| Objective To investigate radiation-induced somatic mutations and variations and provide theoretical basis for clarifying radiation-induced genetic changes and long-term effects by whole-genome sequencing analysis of the genetic variations of the victim of the "5.7" 192Ir radiation accident in Nanjing. Methods Normal back skin tissue, irradiated bone and soft tissues, and peripheral blood were collected from the victim 2 047 days post-irradiation. DNA of these samples was extracted and sequenced with high-throughput genomics and analyzed by bioinformatics method. The genetic variations of between irradiated and normal tissues were compared. Results Compared with normal back skin tissue, there are large amounts of genetic variations in the irradiated bone and soft tissues and peripheral blood, including base substitution (transition, transversion), small insertion, small deletion, copy number variation (gain, loss) and structure variation (large deletion, large duplication, inversion, intra-chromosomal translocation, inter-chromosomal translocation). There were 10 666 genetic variations in the irradiated bone and soft tissues and 11 233 genetic variations in peripheral blood, where thousands of genes were involved in. These variations occurred in the exons, introns, UTR'3, UTR'5, splicing sites, within 5 kb upstream of transcription initiation site, within 5 kb downstream of transcription termination site, ncRNA and intergenic region. All chromosomes had genetic variations. Conclusions There were a large number of genetic variations in the irradiated tissues and blood of the victim at 2 047 days after irradiation, which may affect the body function and cause the long-term effects. |
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