| Xi Cong,Zhao Hua,Lu Xue,Cai Tianjing,Tian Mei,Liu Qingjie.Screening of radiosensitive lipid metabolites in rat small intestine after total body irradiation with 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2021,41(3):172-177 |
| Screening of radiosensitive lipid metabolites in rat small intestine after total body irradiation with 60Co γ-rays |
| Received:October 15, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.03.003 |
| KeyWords:Ionizing radiation Enteropathy Lipidomics Metabolic pathway |
| FundProject:国家自然科学基金（81573081） |
| Author Name | Affiliation | E-mail | | Xi Cong | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Zhao Hua | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Cai Tianjing | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Mei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | liuqingjie@nirp.chinacdc.cn |
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| Abstract:: |
| Objective To screen radiosensitive lipid metabolites in rat small intestine and analyze their metabolic pathways, in order to provide scientific basis for radiation enteropathy biomarkers. Methods The total body irradiation of 60Co γ rays was performed to rats with different doses of 0, 1, 2, 3, 5 and 8 Gy. The changes of lipids in small intestine were studied by targeted lipidomics method based on liquid chromatography coupled mass spectrometry (LC-MS). Results Fifteen lipids in small intestine were screened as radiosensitive metabolites at 3 d after irradiation, including 4 up-regulated lipids and 11 down-regulated lipids(t=-6.395, 5.998, 5.836, -5.503, -5.449, -5.422, 4.841, 4.802, 4.621, 4.457, 4.426, 4.373, 4.110, 3.945, 3.902, P<0.05 and FDR < 0.05).The metabolic pathways of sphingolipid, glycerophosphoplipid were significantly enriched. Four phosphatidyl serines (PS)increased while 1 phosphatidic acid(PA), 2 sphingomyelins(SM) and 4 fatty acids(FA)decreased in a good dose-response manner(R2>0.80,P<0.05), which were more potential radiation enteropathy biomarkers. Conclusions Lipid metabolites in rat small intestine were significantly changed after the rat was total body irradiated with 60Co γ-rays.Eleven lipids with good dose-response relationship were more potential to be radiation enteropathy biomarkers. |
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