| Chen Yuzhong,Zhao Lin,Gu Jia,Du Yafang,Li Yunhong,Duan Guangxin,Qin Liqiang,Xu Jiaying.Lactoferrin improved radioactive lung damage by regulating HMGB1/TLR4 inflammation[J].Chinese Journal of Radiological Medicine and Protection,2021,41(3):161-165 |
| Lactoferrin improved radioactive lung damage by regulating HMGB1/TLR4 inflammation |
| Received:November 09, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.03.001 |
| KeyWords:Lactoferrin Inflammatory cytokine Radiation Lung injury |
| FundProject:国家自然科学基金（81673101，81703159，81973024，82073482）；中国博士后科学基金（2015M571889）；江苏省高等学校自然科学研究重大项目（18KJA310006）；江苏省博士后科学基金（1601121C） |
| Author Name | Affiliation | E-mail | | Chen Yuzhong | State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China | | | Zhao Lin | State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China | | | Gu Jia | State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China | | | Du Yafang | Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, China | | | Li Yunhong | Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, China | | | Duan Guangxin | State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China | | | Qin Liqiang | Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, China | | | Xu Jiaying | State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China | xujiaying@suda.edu.cn |
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| Abstract:: |
| Objective To investigate the protective effect of lactoferrin(Lf) on lung injury in mice exposed to irradiation. Methods C57BL/6 J mice were randomly divided into control group, 15 Gy irradiation group (IR group) and lactoferrin combined 15 Gy irradiation group (Lf+IR group), with 5 mice in each group. The mice in the Lf+15 Gy group drank lactoferrin solution (10 mg/ml) from 3 days before irradiation and contained the whole experiments. Then, single chest 15 Gyirradiation was performed both in the IR and Lf+IR groups. The body weight and other characteristics were monitored during the experiment. The mice were killed at day 14 after irradiation. The lung histopathology was observed by HE staining. Serum inflammatory cytokine such as HMGB1, TNF-α, IL-1β and IL-6 was determined by ELISA method. The expression of inflammatory related protein in lung tissue including HMGB1, TLR4, MyD88 and NF-κB were performed by immune histochemistry and Western blot method. Results Compared with the control group, lung weight was significantly increased (t=3.20, P<0.05), pulmonary hyperemia and inflammatory cell infiltration was observed in the IR group. Exposure also significantly increased serum level of TNF-α[(291.80±5.49)vs.(332.25±22.18)pg/ml](t=3.07, P<0.05), up-regulated the expression of inflammatory related protein in lung tissue (t=4.04, 4.78, 3.77, 6.14, P<0.05). Lactoferrin intervention (Lf+IR group) significantly decreased lung weight (t=2.18, P<0.05), alleviated histopathologic changes, decrease serum levels of HMGB1, TNF-α and IL-1β (t=4.67, 2.97, 3.49, P<0.05). On the other hand, lactoferrin intervention decreased the positive cell number of HMGB1 and NF-κB, and down-regulated the protein expression of HMGB1, TLR4, MyD88 and NF-κB in lung tissues, with significant difference with the IR group (t=8.06, 9.80, 3.07, 5.56, P<0.05). Conclusions Lactoferrin plays the protective effect of radiation-induced lung injury through the downregulation of inflammatory response, such as HMGB1/TLR4/MyD88/NF-κB signaling pathway. |
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