| Zhang Xun,Zhang Nan,Guo Yanru,Liu Qi,Zhou Xue,Mi Yingying,Yang Kang,Zhu Dan.Effect of Siwu decoction on the radiation injury of Zebrafish blood system[J].Chinese Journal of Radiological Medicine and Protection,2020,40(11):820-825 |
| Effect of Siwu decoction on the radiation injury of Zebrafish blood system |
| Received:April 21, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.11.002 |
| KeyWords:Zebra fish Siwu decoction Blood system Irradiation |
| FundProject:2020-09-30 |
| Author Name | Affiliation | E-mail | | Zhang Xun | Key Laboratory of Ethnomedicine Ministry of Education&School of Pharmacy, Minzu University of China, Beijing 100081, China | | | Zhang Nan | Key Laboratory of Ethnomedicine Ministry of Education&School of Pharmacy, Minzu University of China, Beijing 100081, China | | | Guo Yanru | Key Laboratory of Ethnomedicine Ministry of Education&School of Pharmacy, Minzu University of China, Beijing 100081, China | | | Liu Qi | Key Laboratory of Ethnomedicine Ministry of Education&School of Pharmacy, Minzu University of China, Beijing 100081, China | | | Zhou Xue | Key Laboratory of Ethnomedicine Ministry of Education&School of Pharmacy, Minzu University of China, Beijing 100081, China | | | Mi Yingying | Key Laboratory of Ethnomedicine Ministry of Education&School of Pharmacy, Minzu University of China, Beijing 100081, China | | | Yang Kang | Key Laboratory of Ethnomedicine Ministry of Education&School of Pharmacy, Minzu University of China, Beijing 100081, China | | | Zhu Dan | Key Laboratory of Ethnomedicine Ministry of Education&School of Pharmacy, Minzu University of China, Beijing 100081, China | zhudan@muc.edu.cn |
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| Abstract:: |
| Objective To observe the effect of Chinese herbal compound Siwu decoction (SW) on radiation injury of zebra fish blood system, and to clarify whether zebra fish is a suitable model for this study. Methods Totally 72 of 4-month-old adult male zebra fish (Danio Rerio) with body weight ranging from 0.14 to 0.20 g were used in all the experiments, where 36 zebra fish were taken to observe the hemodynamic changes after radiation, and the other 36 zebra fish were used to study the intervention effect of Siwu decoction on the blood injury after radiation. Zebra fish was irradiated with 20 Gy of 60Co gamma-rays at a dose rate of 97.33 cGy/min. For the hemodynamic study, the peripheral blood and whole kidney marrow cells (WKMCs)of irradiated zebra fish and non irradiated control were collected on days 7, 14 and 30 after irradiation, respectively, and the cell number was detected by flow cytometry. For the Siwu decoction treatment, 36 zebra fish were randomly divided into non-irradiation group, irradiation group, SW2 000 group (Siwu decoction was diluted by 2 000-fold) and SW5 000 group (Siwu decoction was diluted by 5 000-fold). Siwu decoction was given on the second day after irradiation. Then the peripheral blood and WKM cells of zebra fish were collected at 7 d after irradiation for further analysis. ResultsAfter irradiation, the dynamic of blood development of zebra fish were obviously changed with the most blood cell ablation at 7 d post-irradiation. The total number of peripheral blood cells and WKM cells decreased by 26% and 52%(t=4.535, 28.987, P<0.05), the number of myeloid monocytes, lymphocytes, and red blood cells in the WKM decreased by 46%, 79% and 33%(t=18.457, 66.900, 9.872, P<0.05), and the number of precursor cells decreased by 49% (P>0.05). The numbers of these blood cells were partly recovered at 14 d after irradiation and returned to the normal level at 30 d after irradiation. In comparison with irradiation alone group, the numbers of WKM cells, myeloid monocytes, progenitor cells, lymphocytes and red blood cells increased by 57%,125%, 81% and 35%, respectively (t=12.128, 21.594, 15.473, 4.594,P<0.05),and peripheral red cells did not increase significantly in the SW5 000 group after irradiation. However, SW2 000 had no significant influence on radiation injury of zebra fish blood cells (P>0.05). Conclusions Radiation injury of the blood system of zebra fish could be recovered at a month after irradiation, and this radiation damage could be partly intervened by Siwu decoction at a suitable concentration, which provides a reference for the study of blood system regulation of traditional Chinese medicine. |
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