| Weng Xiaokun,Hu Lijun,Sun Fei,Wang Jianlin,Xie Kai,Kong Ze,Zhou Mengyun,Gao Liugang,Yu Jingping.Down-regulation of VEGFA increases the radio sensitivity of esophageal cancer ECA-109 cell[J].Chinese Journal of Radiological Medicine and Protection,2020,40(11):813-819 |
| Down-regulation of VEGFA increases the radio sensitivity of esophageal cancer ECA-109 cell |
| Received:July 20, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.11.001 |
| KeyWords:Esophageal cancer Vascular endothelial growth factor A Radiosensitivity DNA damage repair |
| FundProject:国家自然科学基金（11705095）；江苏省"333工程"第二层次人才科研资助项目（BRA2019025） |
| Author Name | Affiliation | E-mail | | Weng Xiaokun | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, 213003, China | | | Hu Lijun | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, 213003, China | | | Sun Fei | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, 213003, China | | | Wang Jianlin | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, 213003, China | | | Xie Kai | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, 213003, China | | | Kong Ze | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, 213003, China | | | Zhou Mengyun | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, 213003, China | | | Gao Liugang | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, 213003, China | | | Yu Jingping | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, 213003, China | yujingping700420@sina.com |
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| Abstract:: |
| Objective To observe the effect and underlying mechanism of down-regulation of VEGFA on the radiosensitivity of esophageal cancer ECA-109 cells. Methods Esophageal cancer cells were divided into four groups: sh-VEGFA group, vector control group, X-ray plussh-VEGFA group and X-ray plus vector group. The expressions of VEGFA gene and protein were detected by qPCR and Western blot, respectively. Cell proliferation and survival was measured by CCK8 assay and cloning formation, respectively. Cell apoptosis was detected by flow cytometry, and γ-H2AX foci were detected by immune-fluorescence assay. Results Compared with the vector group, the expression of VEGFA gene was decreased in sh-VEGFA group (t=11.98,P<0.05), and the expression of VEGFA protein was also reduced(t=12.38,P<0.05). After VEGFA being down-regulated, the cell proliferation(A450)was obviously inhibited(t=2.78,7.25,21.93,13.21,P<0.05), and the cell clone formation of the sh-VEGFA group was significantly decreased so that D0,Dqand SF2 of sh-VEGFA group were decreased(t=5.83,8.56,7.68, P<0.05), and SERD0 and SERDq were increased. Compared with the vector group, the apoptosis rate in the sh-VEGFA group and the X-ray group was significantly increased and further increased in the sh-VEGFA plus X-ray group(t=17.63,22.48,33.87,P<0.05),and the number of γ-H2AX foci in both sh-VEGFA and vector groups were significantly increased within 2 h after X-ray irradiation. At 24 h after irradiation, the number of γ-H2AX foci returned to normal level in the vector group but remained at a higher level in the sh-VEGFA group (t=7.00,P<0.05). Conclusions Down-regulation of VEGFA inhibits the proliferation and colony formation, promotes apoptosis and hence increases the radiosensitivity of esophageal carcinoma cells via a pathway related to DNA damage repair. |
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